You Position: Home > Paper
Inhibitory effects of AcSDKP on proliferation of human bone marrow mesenchymal stem cells in vitro
( views:85, downloads:0 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.2006.02.003
- Key Word:
- AcSDKP;增殖;抑制;间充质干细胞;骨髓
Abstract： M乙酰基-丝氨酰-天冬氨酰-赖氨酰-脯氨酸(Macetyl-seryl-aspartyl-lysyl-proline,AcSDKP)是一种具有生理调控活性的四肽因子,对造血干/祖细胞增殖具有抑制作用.本研究采用集落形成实验、甲基偶氮唑盐(MTT)比色法、细胞分裂指数测定等方法,考察了AcSDKP对体外培养的人骨髓间充质干细胞(mesenchymal stem cell,MSC)增殖的影响.结果显示,在AcSDKP浓度为1×10-12mol/L～1×10-9mol/L的培养体系中,人骨髓MSC集落生成率和大小、活力细胞数和分裂指数均降低,最大效应浓度为1×10-11mol/L.以上实验结果表明,在体外培养条件下,一定浓度的AcSDKP对人骨髓MSC的增殖具有抑制作用.
- [1]侯玲玲,郑敏,王冬梅,等.人骨髓间充质干细胞在成年大鼠脑内的迁移及分化[J].2003,(2).
- [2]Shigeyuki Wakitani,Tomoyuki Saito,Arnold I. Caplan.Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine[J].1995,18(12).
- [3]D, Bonnet,F M, Lemoine,S, Pontvert-Delucq,etc.Direct and reversible inhibitory effect of the tetrapeptide acetyl-N-Ser-Asp-Lys-Pro (Seraspenide) on the growth of human CD34+ subpopulations in response to growth factors.[J].1993,82(11).
- [4]J D, Jackson,Y, Yan,C, Ewel,etc.Activity of acetyl-n-ser-asp-lys-pro (AcSDKP) on hematopoietic progenitor cells in short-term and long-term murine bone marrow cultures.[J].1996,24(3).
- [5]M, Guigon,D, Bonnet,R, Césaire,etc.Effects of small peptidic inhibitors of murine stem cells on human normal and malignant cells.[J].1991,628.
- [6]Arthur E. Bogden,Jacques-Pierre Moreau,Christina Gamba-Vitalo,etc.Goralatide (AcSDKP), a negative growth regulator, protects the stem cell compartment during chemotherapy, enhancing the myelopoietic response to GM-CSF[J].1998,76(1).
- [7]E, Frindel,M, Guigon.Inhibition of CFU entry into cycle by a bone marrow extract.[J].1977,5(1).
- [8]Pittenger, Mark F.,Mackay, Alastair M.,Beck, Stephen C.,etc.Multilineage Potential of Adult Human Mesenchymal Stem Cells.[J].1999,284(5411).
- [9]J E, Chisi,C V, Briscoe,E, Ezan,etc.Captopril inhibits in vitro and in vivo the proliferation of primitive haematopoietic cells induced into cell cycle by cytotoxic drug administration or irradiation but has no effect on myeloid leukaemia cell proliferation.[J].2000,109(3).
- [10]J, Li,L, Volkov,L, Comte,etc.Production and consumption of the tetrapeptide AcSDKP, a negative regulator of hematopoietic stem cells, by hematopoietic microenvironmental cells.[J].1997,25(2).
- [11]汪保和,王绮如.小鼠骨髓内皮细胞无血清条件培养液及其超滤组分对粒巨噬系祖细胞生长的影响[J].1999,(6).
- [12]Rousseau A,Michaud A,Corvol P,etc.The hemoregulatory peptide N-acetyl-Ser-Asp-Lys-Pro is a natural and specific substrate of the N-terminal active site of human angiotensin-converting enzyme.[J].1995,270(8).
- [13]Leonid Volkov,Pascale Quéré,Françoise Coudert,etc.The Tetrapeptide AcSDKP, a Negative Regulator of Cell Cycle Entry, Inhibits the Proliferation of Human and Chicken Lymphocytes[J].1996,168(2).
- [14]D, Bonnet,R, Césaire,F, Lemoine,etc.The tetrapeptide AcSDKP, an inhibitor of the cell-cycle status for normal human hematopoietic progenitors, has no effect on leukemic cells.[J].1992,20(2).