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Changes of somatostatin and expression of somatostatin receptor in small intestine and liver tissues during macaque development
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2005.06.008
- Key Word:
- 生长抑素;生长抑素受体;发育;猕猴;小肠;肝脏
Abstract： 探讨在猕猴发育过程中生长抑素(somatostatin,SST)及其受体在肠肝组织的演变规律.通过手术途径获得胚胎6月、新生2 d、新生45 d和成年猕猴的回肠、肝脏、门静脉和外周血等标本.应用放射免疫分析法测定各标本中的SST含量;通过免疫组化方法观察SST在肠、肝组织内的分布;利用原位杂交检测SST受体2(somatostatin receptor 2,SSTR2)的表达.结果显示:(1)胚胎6月的猕猴,小肠内SST含量为(273±16.6)ng/mg蛋白;黏膜隐窝处SST呈弱阳性染色,肌层SST染色阴性.在发育过程中,小肠内SST含量逐渐增加,成年期时达最高水平(120.1±35.3)ng/mg蛋白,较胚胎6月显著增加(P＜0.01).(2)成年小肠黏膜隐窝处及肌间神经丛SST呈强阳性染色.(3)胚胎6月,小肠粘膜上皮可见大量SSTR2表达,成年时SSTR2表达下调,且主要定位于腺上皮隐窝处;胚胎及新生期肌层SSTR2染色阴性,成年时小肠肌间神经丛则可见阳性SSTR2染色.(4)肝脏在发育过程中SST及SSTR2含量逐渐降低;发育的各个时期,小肠组织的SST含量均明显高于肝脏组织含量,门静脉SST水平也始终高于外周血.总之,位于小肠黏膜隐窝处的SST和SSTR2随着发育逐渐增加,来自肠道的SST进入门静脉后迅速被降解.SST阳性的肠肌间神经丛仅在发育成熟后才出现.
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