You Position: Home > Paper
Telomerase: the expression and regulatory mechanisms in preovulatory ovarian granulosa cells
( views:105, downloads:20 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2005.06.007
- Key Word:
- 排卵前期卵巢颗粒细胞;端粒;端粒酶;表达
Abstract： 用端粒酶重复扩增酶联免疫吸附分析法(telomeric repeat amplification protocol-enzymelinkedimmunoadsordent assay,TRAP-ELISA)观察体外培养的大鼠排卵前期卵巢颗粒细胞中端粒酶活性的表达及其影响因素,并用放射免疫分析法(radioimmunoassay,RIA)同步测定培养液中雌二醇(estradiol,E2)、孕酮(progesterone,Po)含量的变化及MTT(四甲基偶氮唑盐)法测定颗粒细胞增殖指数,分析颗粒细胞中端粒酶活性的表达以及端粒酶活性表达的影响因素.本实验中大鼠排卵前期卵巢颗粒细胞中有端粒酶活性表达,且在人绒毛膜促性腺激素(human chorionic gonadotropin,HCG)、卵泡刺激素(follicle-stimulating hormone,FSH)、二丁酰环磷腺苷(dbcAMP)及维拉帕米(verapamil)作用下活性明显升高,而在反义c-myb作用下活性明显降低.RIA测定培养液中雌激素及孕激素含量发现,在verapamil及FSH作用下E2与p0分泌量明显升高,在dbcAMP及HCG作用下分泌量无明显改变,而在反义c-myb作用下分泌量明显降低,在不同作用因素下的端粒酶活性与它相对应的E2及P0分泌量无相关性.MTT法测定显示,反义hTERT能明显抑制颗粒细胞的增殖.由此可以证实,排卵前期卵巢的颗粒细胞中表达有端粒酶活性,其活性受FSH、HCG、verapamil、dbcAMP及癌基因的影响,并且端粒酶活性与颗粒细胞增殖功能相关.
- [1]王孝养,张珍祥,徐永健,等.端粒酶逆转录酶基因反义寡核苷酸抑制肺癌细胞端粒酶活性和诱导细胞凋亡[J].2002,(3).
- [2]刘卫军,丁健.端粒酶活性调节的分子机制[J].2001,(3).
- [3]de Matos MHT,de,Silva JRV,etc.Influences of FSH and EGF on primordial follicles during in vitro culture of caprine ovarian cortical tissue[J].2004,61(9).
- [4]N W, Kim,M A, Piatyszek,K R, Prowse,etc.Specific association of human telomerase activity with immortal cells and cancer.[J].1994,266.
- [5]S, Mussche,L, Leybaert,K, D'Herde.First and second messenger role of calcium. Survival versus apoptosis in serum-free cultured granulosa explants.[J].2000,926.
- [6]Blasco MA.,Keefe DL.,Trimarchi JR.,etc.An essential role for functional telomeres in mouse germ cells during fertilization and early development[J].2002,249(1).
- [7]R, Dahse,W, Fiedler,G, Ernst.Telomeres and telomerase: biological and clinical importance.[J].1997,43.
- [8]J, Xu,X, Yang.Telomerase activity in bovine embryos during early development.[J].2000,63(4).
- [9]Christoph Lengauer.How do tumors make ends meet?[J].2001,98(22).
- [10]R, Levy.Genetic regulation of preimplantation embryo survival.[J].2001,210.
- [11]K M, Eisenhauer,R M, Gerstein,C P, Chiu,etc.Telomerase activity in female and male rat germ cells undergoing meiosis and in early embryos.[J].1997,56.
- [12]A G, Bodnar,M, Ouellette,M, Frolkis,etc.Extension of life-span by introduction of telomerase into normal human cells.[J].1998,279(5349).
- [13]Betts D,Bordignon V,Hill J,etc.Reprogramming of telomerase activity and rebuilding of telomere length in cloned cattle.[J].2001,98(3).
- [14]Murakami T,Yajima A,Kinugawa C,etc.Telomerase activity in normal ovaries and premature ovarian failure.[J].2000,190(3).
- [15]Kotoula V,Papadimitriou CS,Thung SN,etc.Expression of human telomerase reverse transcriptase in regenerative and precancerous lesions of cirrhotic livers.[J].2002,22(1).
- [16]杨秀芝,陈必良,苏明权,等.端粒酶活性在卵巢肿瘤组织中表达的临床意义[J].2000,(4).