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cAMP response-element binding protein participates in the phosphorylated extracellular signal-regulate kinase mediated neuropathic pain

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Author:: No author available

Journal Title:: ACTA PHYSIOLOGICA SINICA

Issue:: 2

DOI:: 10.3321/j.issn:0371-0874.2005.02.005

Key Word:: cAMP反应元件结合蛋白;神经病理性痛;原癌基因c-fos;细胞外信号调节激酶

Abstract：采用行为学、免疫组织化学和Western blot方法,观察鞘内注射细胞外信号调节激酶(extracellular signal-regulate kinase,ERK)信号转导通路阻滞剂对慢性压迫性损伤(chronic constriction injury,CCI)大鼠痛行为及脊髓背角内磷酸化cAMP反应元件结合蛋白(phosphorylated cAMP response-element binding protein,pCREB)和Fos表达变化的影响,探讨ERK/CREB转导通路在神经病理性疼痛中的作用.结果表明,CCI可明显增加双侧脊髓背角pCREB、损伤侧脊髓背角浅层Fos阳性神经元表达,以CCI后3与5 d时尤为显著.鞘内注射促分裂原活化蛋白激酶激酶(mitogen-activated protein kinase kinase,MEK)阻滞剂U0126及ERK反义寡核苷酸在减轻大鼠痛行为的同时,能明显抑制双侧脊髓背角内pCREB的表达,同时,Fos阳性神经元的表达也明显减少.大鼠痛行为及脊髓背角pCREB和Fos的表达在时相上一致.上述结果提示pCREB参与pERK介导的神经病理性疼痛.

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