Induction of rat neural stem cells into oligodendrocyte precursor cells

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FU Sai-li()
HU Jian-Guo()
LI Ying()
YIN Lan()
JIN Jian-qiang()
XU Xiao-Ming()
LU Pei-hua()
Journal Title:
Volume 57, Issue 02, 2005
Key Word:
neural stem cells;oligodendrocyte precusor cells;B 104 neuroblastoma cells;basic fibroblast growth factor

Abstract: We have previously established a culture method to isolate and cultivate neural stem cells (NSCs) derived from the rat embryonic brain and spinal cord. In the present study, we demonstrate that the spinal cord-derived NSCs can be induced to differentiate into oligodendrocyte precursor cells (OPCs) with a combined treatment composed of (1) conditioned medium collected from B 104neuroblastoma cells (B104CM) and (2) basic fibroblast growth factor (bFGF, 10 ng/ml). After induction, over 95% of the cells displayed bipolar or tri-polar morphology and expressed A2B5 and platelet derived growth factor receptor-α (PDGFR-α), markers that are specific for OPCs. Among PDGFR-α positive OPCs, only a few cells expressed glia fibrillary acidic protein (GFAP) and none expressed β-tubulin Ⅲ. In the presence of B 104CM and bFGF, OPCs proliferated rapidly, formed spheres, expanded for multiple passages, and maintained their phenotypic properties. Upon withdrawal of B 104CM and bFGF, these cells differentiated into either O4/GlaC-positive oligodendrocytes (OLs) or GFAP- and A2B5-positive type-2 astrocytes. Our results indicate that NSCs can be induced to differentiate into OPCs that possess properties of self-renewal and differentiation into oligodendrocytes and type-2astrocytes, a property similar to that of O-2A progenitor cells. The OPCs can be maintained in an undifferentiated state over multiple divisions as long as both B 104CM and bFGF are present in the medium. Thus, large quantity of OPCs can be obtained through this method for potential therapeutical interventions for various neurological degenerative diseases.

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