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Effects of lactation, lactation-cessation and lactation-cessation-lactation paradigms on hypothalamic orexin-A immunoreactive neurons in rats
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2004.06.005
- Key Word:
- 神经生物学;下丘脑;胖素A;免疫组织化学;图像分析;哺乳
Abstract： 为观察下丘脑胖素A在哺乳期摄食增加和能量代谢中的作用,本研究采用脑连续切片之免疫组织化学和图像定量分析技术,对分娩后第12天非哺乳、持续哺乳、持续哺乳后禁哺乳过夜和持续哺乳-禁哺乳后再急性哺乳大鼠下丘脑胖素A免疫反应神经元的免疫反应性进行了观察和半定量分析.结果表明,分娩后持续哺乳11d,大鼠的日摄食量较同期分娩的非哺乳大鼠明显增加(180%),一夜禁哺乳则明显降低哺乳大鼠的日摄食量(45%);哺乳12 d,大鼠下丘脑胖素A免疫反应神经元的数目和平均染色强度较非哺乳大鼠明显增加(P＜0.001,P＜0.05);禁哺乳过夜(15 h)明显降低哺乳大鼠胖素A免疫反应神经元的数目和平均染色强度(P＜0.001, P＜0.05),与非哺乳大鼠比较无明显差异;禁哺乳过夜后再急性哺乳2 h明显增加禁哺乳大鼠胖素A免疫反应神经元的数目和平均染色强度(P＜0.001,P＜0.05),急性哺乳5 h后,虽亦明显增加禁哺乳大鼠胖素A免疫反应性(P＜0.05),但与急性哺乳2 h比较作用减弱.上述结果表明,持续哺乳和禁乳后再哺乳均导致下丘脑胖素A明显增加,提示哺乳期胖素A可能表达上调并可能与哺乳期摄食增加有关,且吸乳动作与下丘脑胖素A样神经元之间可能存在某种神经或体液性联系途径.
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