Structural survey of airway sensory receptors in the rabbit using confocal microscopy

( views:53, downloads:0 )
Journal Title:
Volume 56, Issue 02, 2004
Key Word:
airway mechanoreceptors;vagal afferents;visceral sensory nerve;immunohistochemistry;confocal microscopy

Abstract: Information on the morphology of airway receptors is limited. The present study surveys rabbit airway receptors using immunohistochemical and fluorescent labeling to identify their structure with confocal microscopy. Various receptor types were observed to have multiple branches where a parent axon fed several structures. Receptors were located in different layers of the airway, i.e., smooth muscle, lamina propria (submucosa) and the epithelium. Smooth muscle and submucosal receptors were innervated by thick myelinated fibers, while epithelial receptors were supplied by thin-diameter axons. Structures of smooth muscle receptors and some submucosal receptors covered a relatively large area, while epithelial receptors were less extended. In addition, intrapulmonary ganglia were also labeled. Some were closely associated with the axons of smooth muscle receptors.

  • [1]Larsell O. Nerve terminations in the lung of the rabbit. J Comp Neurol 1921; 33: 105-131.
  • [2]Larsell O. The ganglia, plexuses, and nerve-terminations of the mammalian lung and pleura pulmonalis. J Comp Neurol 1922;35: 97-132.
  • [3]Adrian ED. Afferent impulses in the vagus and their effect on respiration. J Physiol 1933; 79: 332-358.
  • [4]Coleridge HM, Coleridge JCG. Reflexes evoked from tracheobronchial tree and lungs. In: Chemiack NS, Widdicombe JG, eds.Handbook of Physiology. 2nd ed. Bethsda: American Physiological Society, 1986: 395-430.
  • [5]Schelegle ES, Green JF. An overview of the anatomy and physiology of slowly adapting pulmonary stretch receptors.Respir Physiol 2001; 125: 17-31.
  • [6]Sant'Ambrogio G, Widdicombe J. Reflexes from airway rapidly adapting receptors. Respir Physiol 2001; 125: 33-45.
  • [7]Lee LY, Pisarri TE. Afferent properties and reflex functions of bronchopulmonary C-fibers. Respir Physio12001; 125: 47-65.
  • [8]Yu J. An overview of vagal airway receptors. Acta Physiol Sin 2002; 54(6): 451-459.
  • [9]Jones AC. Innervation and nerve terminations of the reptilian lung. J Comp Neurol 1925; 40: 371-388.
  • [10]Hunjin R. On the nerve supply of the lung of the mouse, with special reference to the structure of the peripheral vegetative nervous system. J Comp Neurol 1956;105:587-625.
  • [11]Düring MV, Andres KH, Iravani J. The fine structure of the pulmonary stretch receptor in the rat. Z Anat Entwicklungsgesch 1974; 143: 215-222.
  • [12]Baluk P, Gabella G. Afferent nerve endings in the tracheal muscle of guinea-pigs and rats. Anat Embryol (Berl.) 1991; 183:81-87.
  • [13]Krauhs JM. Morphology of presumptive slowly adapting receptors in dog trachea. Anat Rec 1984; 210: 73-85.
  • [14]Hirsch EF, Kaiser GC, Barner HB, Cooper T, Rams JJ.Innervation of the mammalian lung. I. The afferent receptors.Arch Pathol 1968; 85: 51-61.
  • [15]Fisher AWF. The intrinsic innervation of the trachea. J Anat (London) 1964; 98: 117-124.
  • [16]Larsell O, Dow RS. The innervafion of the human lung. Am J Anat 1933; 52: 125-146.
  • [17]Elftman AG. The afferent and parasympathetic iunervation of the lungs and trachea of the dog. Am J Anat 1943; 72: 1-28.
  • [18]Yamamoto Y, Atoji Y, Suzuki Y. Nerve endings in bronchi of the dog that react with antibodies against neurofilament protein. J Anat 1995; 187: 59-65.
  • [19]Yamamoto Y, Atoji Y, Suzuki Y. Calretinin immunoreactive nerve endings in the trachea and bronchi of the rat J Vet Med Sci 1999; 61: 267-269.
  • [20]Wang YF, Yu J. Na+/K+-ATPase as a marker for detecting pulmonary sensory receptors. Acta Physiol Sin 2002; 54(5):390-394.
  • [21]Widdicombe JG. Afferent receptors in the airways and cough.Respir Physiol 1998; 114: 5-15.
  • [22]Yu J, Wang YF, Zhang JW. Structure of slowly adapting pulmonary stretch receptors in the lung periphery. J Appl Physiol 2003; 95: 385-393.
  • [23]Yamamoto Y, Hayashi M, Atoji Y, Suzuki Y. Vagal afferent nerve endings in the trachealis muscle of the dog. Arch Histol Cytol 1994; 57: 473-480.
  • [24]Yu J. Spectrum of myelinated pulmonary afferents. Am J Physiol Regul Integr Comp Physio12000; 279: R2142-R2148,
  • [25]Widdicombe JG. Airway receptors. Respir Physio12001; 125:3-15.
  • [26]Sant'Ambrogio G, Remmers JE, de Groot WJ, Callas G,Mortola Jl. Localization of rapidly adapting receptors in the trachea and main stem bronchus of the dog. Respir Physiol 1978; 33: 359-366.
  • [27]Sellick H, Widdicombe JG. Stimulation of lung irritant receptors by cigarette smoke, carbon dust, and histamine aerosol. J Appl Physiol 1971; 31: 15-19.
  • [28]Baluk P, Nadel JA, McDonald DM. Substance P-immunoreactive sensory axons in the rat respiratory tract: a quantitative study of their distribution and role in neurogenic inflammation.J Comp Neurol 1992; 319: 586-598.
  • [29]Yu J, Zhang JW. A single slowly adapting pulmonary stretch receptor (SAR) has multiple encoders. FASEB J 2003; 17:821(Abstract).
  • [30]Zegorodnyuk VP, Brookes SJ. Transduction sites of vagal mechanoreceptors in the guinea pig esophagus. J Neurosci 2000;20: 6249-6255.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615