You Position: Home > Paper
Osteopontin enhances migratory ability of cultured aortic adventitial fibroblasts from spontaneously hypertensive rats
( views:252, downloads:16 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2004.01.004
- Key Word:
- 主动脉;外膜;成纤维细胞;骨桥蛋白;高血压;细胞迁移;重构
Abstract： 血管外膜成纤维细胞迁移参与形成新生内膜是一些血管疾病的共同发病过程.研究高血压动物模型的外膜成纤维细胞是否与对照组不同将有利于阐述高血压血管重塑的机制.本实验比较自发性高血压大鼠(spontaneously hypertensive rats,SHR)与正常对照大鼠(Wistar-Kyoto rats,WKY)的血管外膜成纤维细胞在体外培养条件下迁移能力的差别,并对其机制进行了探讨.采用大鼠胸主动脉的培养血管外膜成纤维细胞,用Transwell技术测定培养细胞的迁移能力.用实时定量PCR技术检测mRNA表达.结果表明,在血清和bFGF趋化作用下,SHR培养血管外膜成纤维细胞的迁移活性显著强于WKY(每个视野平均迁移细胞数目,血清:35.20±5.26 vs 22.2±3.27,P＜0.05;bFGF:30 23±4.54vs 19.20±4.47,P＜0.05).进一步研究发现,SHR培养血管外膜成纤维细胞中的骨桥蛋白(osteopontin,OPN)mRNA水平显著高于WKY(1863.23±43.91 vs 326.24±68.29,P＜0.01).反义OPN(100μmol/L)对血清诱导的SHR血管外膜成纤维细胞迁移有抑制作用(每个视野平均迁移细胞数目38.60±5.98 vs 26.61±3.84,P＜0.05).而正义及错配义OPN组均无此效应.反义OPN对SHR细胞迁移的抑制作用呈浓度依赖性.上述结果证实SHR培养血管外膜成纤维细胞的迁移能力强于WKY,OPN在细胞迁移中可能起重要作用.
- [1]孙爱军,高平进,刘建军,等.用差异显示PCR法筛选与血管外膜细胞表型转化相关的基因[J].2001,(6).
- [2]D L, Zhu,T, Herembert,P, Marche.Increased proliferation of adventitial fibroblasts from spontaneously hypertensive rat aorta.[J].1991,9.
- [3]H, Abedi,I, Zachary.Signalling mechanisms in the regulation of vascular cell migration.[J].1995,30.
- [4]J P, Heath,B F, Holifield.Cell locomotion: new research tests old ideas on membrane and cytoskeletal flow.[J].1991,18.
- [5]T L, Yue,P J, McKenna,E H, Ohlstein,etc.Osteopontin-stimulated vascular smooth muscle cell migration is mediated by beta 3 integrin.[J].1994,214.