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Effect of protein kinase C on KV channel in rat bronchial smooth muscle
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.2003.02.004
- Key Word:
- 支气管;平滑肌细胞;电压依赖性延迟整流钾通道;蛋白激酶C
Abstract： 用全细胞膜片钳、 Western印迹法和逆转录-PCR技术, 观察蛋白激酶C (protein kinase C, PKC)对大鼠支气管平滑肌细胞(bronchial smooth muscle cells, BSMCs)电压依赖性延迟整流钾通道(KV)活性及其亚型KV1.5表达的影响.结果为: (1) PKC激活剂豆蔻酰佛波醇乙酯(phorbol 12-myristate 13-acetate, PMA)显著抑制急性分离大鼠BSMCs的 KV通道电流, 该效应被PKC阻断剂Ro31-8220显著抑制; (2)PMA显著抑制体外培养大鼠BSMCs的KV1.5 mRNA和蛋白质的表达, 该效应被Ro31-8220显著抑制. 上述观察结果提示, PKC活化可抑制大鼠BSMCs的KV通道电流活性, 下调KV1.5亚型的表达水平.
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