Regulatory peptides modulate ICAM-1 gene expression and NF-κB activity in bronchial epithelial cells

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Author:
TAN Yu-rong()
QIN Xiao-Qun()
GUAN Cha-Xiang()
ZHANG Chang-Qing()
LUO Zi-qiang()
SUN Xiu-Hong()
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 55, Issue 02, 2003
DOI:
Key Word:
intrapulmonary regulatory peptides;intercellular adhesion molecule-1 (ICAM-1);nuclear factor κB (NF-κB);bronchial epithelial cells

Abstract: Intercellular adhesion molecule-1 (ICAM-1) is an important adhesion molecule leading to adhesion between cells; NF-ΚB, being universally distributed in the organism, is an important nuclear transcription factor leading to a rapid response to the stimuli. Line of evidence have shown that ICAM-1 transcription and NF-ΚB activation is an important step of inflammatory reaction. To testify that intrapulmonary regulatory peptides modulate inflammatory lesion of bronchial epithelial cells (BECs) through their effect on ICAM-1 expression and nuclear factor ΚB (NF-ΚB) activation, we used immunocytochemistry, RT-PCR, and electrophoretic mobility-shift assay (EMSA) to determine the ICAM-1 expression and NF-ΚB activity in BECs. The effects of NF-ΚB inhibitor MG-132 on ICAM-1 expression were also observed. The results showed that vasoactive intestinal peptide (VIP) and epidermal growth factor (EGF) decreased ICAM-1 expression in O3-stressed BECs, while endothelin-1 (ET-1) and calcitonin gene-related peptides (CGRP) increased ICAM-1 expression in resting BECs. MG-132 blocked ICAM-1 expression induced by O3, ET-1 and CGRP. The results obtained by using EMSA confirmed that VIP and EGF restrained the activation of NF-ΚB in O3-stressed BECs; CGRP and ET-1 promoted activation of NF-ΚB. These observations indicate that VIP and EGF abated the injury by means of down-regulatory effects on ICAM-1 transcription and NF-ΚB activation, while ET-1 and CGRP enhanced the inflammation reaction by an up-regulatory effect. It is suggested that a developing and intensive airway inflammation correlates closely with a persistent expression of ICAM-1 and repeated activation of NF-ΚB.

  • [1]Tan YR (谭宇蓉), Qin XQ, Guan CX, Zhang CQ. Effects of regulatory peptides on adhesion of eosinophil to bronchial epithelial cells. Acta Physiol Sin (生理学报) 2002; 54(1):43-46 (Chinese, English abstract).
  • [2]Qin XQ (秦晓群), Sun XH, Zhang CQ. Cytoprotective effects of vasoactive intestinal peptide on rabbit bronchial epithelial cells damaged by ozone exposure. Chin J Appl Physiol (中国应用生理杂志) 1998;14:250-253 (Chinese, English abstract).
  • [3]Lin JM, Gao J. Upregulation of cell adhesion molecule by endothelin-1 on human mesangial cells in vitro. Chin J Nephrol April 1997;13(2):67-69.
  • [4]Goldie RG, Henry PJ. Endothelins and asthma. Life Sci 1999;65(1):1-15.
  • [5]Nagakawa O, Ogasavara M, Murata J, Fuse H, Saiki I. Effect of prostatic neuropeptides on migration of prostate cancer cell lines. Int J Urol 2001;8(2):65-70.
  • [6]Qin XQ (秦晓群), Sun XH, Zhang CQ. Tecknique of enzyme digestion adding brushing for isolating bronchial epithelial cells. Bull Hunan Med Univ (湖南医科大学学报) 1999;24(5): 74-77.
  • [7]Natali PG, Hamby CV, Felding-Habermann B et al. Clinical significance of α v β3 integrin and intercellular adhesion molecule-1 expression in cutaneous malignant lesions. Cancer Res 1997;57(15):1554-1560.
  • [8]Launoit Y, Audette M, Pelezar H, Plaza S, Baert JL. The transcription of the intercellular adhesion molecule-1 is regulated by Ets transcription factors. Oncogene 1998;16:2065-2073.
  • [9]Xia YF, Liu LP, Zhong CP, Geng JG. NF-kappaB activation for constitutive expression of VCAM-1 and ICAM-1 on B lymphocytes and plasma cells. Biochem Biophys Res Commun 2001; 289(4):851-856.
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