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Effect of ginkgolide B on L-type calcium current and cytosolic [Ca2+]i in guinea pig ischemic ventricular myocytes
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2003.01.007
- Key Word:
- 银杏苦内酯B;心室肌细胞;缺血;L-型钙电流;细胞内游离钙
Abstract： 应用全细胞膜片钳及激光共聚焦技术, 研究银杏苦内酯B(ginkgolide B,GB)对豚鼠心室肌细胞L-型钙电流及胞内游离钙的作用, 并探讨GB心肌保护作用的机制.实验结果显示, 在指令电压为0 mV时, GB对生理状态下豚鼠心室肌细胞L-型钙电流无明显作用.在模拟缺血状态下, L-型钙峰值电流减小37.71%, 但加入1 μmol/L GB后, 可逆转缺血引起的L-型钙电流的降低, 与缺血对照组比较, 有显著性差异(P<0.05).1 μmol/L GB能使由于模拟缺血而上移的L-型钙电流-电压曲线回复正常.在生理状态下, 0.1、 1、 10 mol/L GB 分别使心肌细胞内游离钙降低10.58% (n=12)、 17.27%(n=12)、 16.35% (n=10), 与对照组相比有非常显著性差异.模拟缺血液灌流12 min时, 细胞内游离钙浓度增加20.15%, 在模拟缺血液中分别加入1 μmol/L nifedipine或5 mmol/L NiCl2, 结果显示: 模拟缺血液灌流12 min, 与正常对照组相比细胞内钙分别增加18.18% (P>0.05)与11%(P<0.05).在模拟缺血液中加入1 mol/L GB灌流12 min时细胞内钙仅增加9.60% (n=12,P<0.001), 与缺血对照组相比有显著性差异(P<0.05). 结果表明, GB可逆转模拟缺血造成L-型钙电流的降低, 同时可部分减轻由于缺血所造成的细胞内钙的超载.
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