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The expression of nestin in ischemia-injured brain of adult rat

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Author:: No author available

Journal Title:: ACTA PHYSIOLOGICA SINICA

Issue:: 4

DOI:: 10.3321/j.issn:0371-0874.2002.04.004

Key Word:: 脑缺血;荧光双标;激光共聚焦扫描显微镜;巢蛋白 (nestin)

Abstract：应用免疫组化和免疫荧光双标技术结合激光共聚焦扫描显微镜, 观察缺血性脑损伤后脑内nestin的表达及其细胞类型.实验观察结果为, 再灌后1天, 在缺血中心区可见nestin阳性突起; 再灌后3天和1周时,除缺血中心区外,周边Ⅰ、Ⅱ、Ⅲ区均有nestin大量表达; 而2周时仅限于周边Ⅰ区大量表达.激光共聚焦扫描显微镜观察到,再灌后3天, 周边Ⅰ区nestin阳性突起主要与GFAP共存; 2周时,nestin阳性突起变粗、变长,并与NSE的共存明显增多.上述研究结果提示,脑缺血可诱导大鼠脑缺血区域表达nestin,该表达可能与神经细胞的修复有关.

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