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Protective effect of low concentration endothelin-1 on the reactive oxygen-induced inhibition of pulmonary surfactant lipid synthesis
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.2002.02.001
- Key Word:
- 内皮素-1;肺表面活性物质;CTP;磷酸胆碱二胞苷酰基转移酶;活性氧
Abstract： 在离体肺组织培养模型上观察低浓度(1～100 pmol/L)内皮素-1 (endothelin-1,ET-1)对活性氧所致肺表面活性物质(pulmonary surfactant,PS)脂质合成障碍及PS脂质主要组分磷脂酰胆碱合成限速酶CTP: 磷酸胆碱二胞苷酰基转移酶(phosphorylcholine cytidylyltransferase,CCT)活性的影响.结果显示: (1) 黄嘌呤-黄嘌呤氧化酶超氧阴离子生成系统呈剂量依赖性地降低肺组织 3H-胆碱的掺入量; (2) ET-1可减轻活性氧所致 3H-胆碱掺入量的减少和肺组织丙二醛含量的增高; 但对肺组织超氧化物歧化酶、过氧化氢酶及总抗氧化能力无明显影响; (3) ET-1可分别提高和降低肺组织细胞微粒体和胞浆的CCT活性,并可减轻活性氧所致肺微粒体CCT活性的降低.结果表明,低浓度 ET-1具有保护肺微粒体的CCT活性、减轻氧化性肺损伤所致PS合成障碍的作用,其保护机制并非通过影响肺组织内源性抗氧化系统而实现.
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