Prokaryotic expression and purification of human hepatic stimulator substance

( views:, downloads: )
Journal Title:
Volume 54, Issue 01, 2002
Key Word:


  • [1]LaBrecque DR,Pesch.Preparation and partial characterization of hepatic regenerative stimulator substance (HSS) from rat liver.J Physiol,1975,248,273~284.
  • [2]Francavilla A,Ove P,Polimeno L,Coetzee M,Makowka L,Rose J,Van Thiel DH,Starzl TE.Extraction and partial purification of a hepatic stimulatory substance in rats,mice and dog.Cancer Res,1987,47:5600~5605.
  • [3]Fleig WE,Hoss G.Partial purification of rat hepatic stimulator substance and characterization of its action on hepatoma cells and normal hepatocytes.Hepatology,1989,9:240~248.
  • [4]Liakos AA,Mykoniatis MG,Kokala ME,Papadimitriou DG,Liatsos GD.Levels of hepatic stimulator substance in liver regenerating process of partially hepatectomized rats pretreated with single dose of carbon tetrachloride.Dig Dis Sci,1999,44:1046~1053.
  • [5]An W,Liu XJ,Lei TG,Dai J,Du GG.Growth induction of hepatic stimulator substance in hepatocytes through its regulation on EGF receptors.Cell Res,1999,9:37~49.
  • [6]Dai J (戴杰),An W (安威),Gao DC (高鼎成),Chen L (陈莉).Influence of hepatic stimulator substance on p21ras expression in human hepatic carcinoma cells BEL-7402.Acta Physiol Sin (生理学报),2000,52(3):225~229 (Chinese,English abstract).
  • [7]Chen L (陈莉),An W (安威),Tan X (谭信),Gao DC (高鼎成),Dai J (戴杰).Phosphorylation of hepatic stimulator substance on mitogen-activated protein kinase in BEL-7402 hepatoma cells.Chin J Hepatol,2001,9:22~24 (Chinese,English abstract).
  • [8]Mosmann T.Rapid colormetric assay for cellular growth and survival:application and cytotoxicity assays.J Immunol Methods,1983,65:55~63.
  • [9]Mei MH,An W,Zhang BH,Shao Q,Gong DZ.Hepatic stimulator substance protects against acute liver failure induced by carbon tetrachloride poisoning in mice.Hepatology,1993,17(4):638~644.
  • [10]Villamon E,Gozalbo D,Martinez JP,Gil ML.Purification of a biologically active recombinant glyceraldehyde 3-phosphate dehydrogenase from Candida albicans.FEMS Microbiol Lett,1999,179(1):61~65.
  • [11]Davis GD,Elisee C,Newham DM,Harrison RG.New fusion protein systems designed to give soluble expression in Escherichia coli.Biotechnol-Bioeng,1999,65:382~388.
  • [12]Luo ZH,Hua ZC.Increased solubility of glutathione s-transferase-P16 (GST-p16) fusion protein by co-expression of chaperones groes and groel in Escherichia coli.Biochem Mol Biol Int,1998,46(3):471~477.
  • [13]Harkonen T,Hovi T,Roivainen M.Expression of Coxsackiexirus B4 proteins VP0 and 2C in Escherichia coli and generation of virus protein recognizing antisera.J Virol Methods,1997,69(1-2):147~158.
  • [14]Shen SH.Multiple joined genes prevent product degradation in Escherichia coli.Proc Natl Acad Sci USA,1984,81:4627~4631.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615