You Position: Home > Paper
Increased resistance against oxidant-induced injury in the rat vascular smooth muscle cells transfected with human heme oxygenase-1 gene
( views:147, downloads:10 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2002.01.006
- Key Word:
- 血红素加氧酶;逆转录病毒载体;活性氧;血管平滑肌
Abstract： 本实验构建含人血红素加氧酶-1(hHO-1)基因的逆转录病毒载体XM-6/hHO-1, 将其导入离体培养的大鼠血管平滑肌细胞(vascular smooth muscle cells, VSMC), 观察外源性hHO-1基因在VSMC内的表达及其抗活性氧损伤作用.结果表明: (1) hHO-1基因可在靶细胞中明显表达, 转染VSMC的HO-1蛋白表达和HO酶活性分别比非转染细胞高1.8倍和2.0倍; (2)转染hHO-1的VSMC可对抗大剂量H2O2对细胞的损伤作用, 表现为细胞存活率增加和乳酸脱氢酶(LDH)漏出减少,上述保护作用可被HO特异性抑制剂锌原卟啉IX (Zinc-protoporphyrin IX, ZnPP-IX)所阻断.研究结果提示, 外源性HO-1的过量表达可增加VSMC对抗氧化损伤的能力.
- [1]郑新程,安威,白景香,等.人SOD1基因在大鼠血管平滑肌细胞的表达及对抗氧自由基损伤的作用[J].1999,(2).
- [2]刘俊昌,张敏,郑新程,等.内毒素引起的乳鼠心肌细胞血红素加氧酶-1基因的表达[J].2001,(1).
- [3]Nick HS,Agarwal A.Renal response to tissue injury: lessons from heme oxygenase-1 GeneAblation and expression.[J].2000,11(5).
- [4]L J, Wang,T S, Lee,F Y, Lee,etc.Expression of heme oxygenase-1 in atherosclerotic lesions.[J].1998,152.
- [5]K, Senior.Gene therapy: a rocky start to the new millennium.[J].2000,6(3).
- [6]Pacilio C,Romano G,Giordano A.Gene transfer technology in therapy: current applications and future goals.[J].1999,17(4).
- [7]Witztum J L,Steinberg D.Role of oxidized low density lipoprotein in atherogenesis.[J].1991.
- [8]Jari Koistinaho,Susanna Miettinen,Riitta Keinänen,etc.Long-term Induction of Haem Oxygenase-1 (HSP-32) in Astrocytes and Microglia Following Transient Focal Brain Ischaemia in the Rat[J].1996,8(11).
- [9]M. Scarpa,D. Cournoyer,D.M. Muzny,etc.Characterization of recombinant helper retroviruses from moloney-based vectors in ecotropic and amphotropic packaging cell lines[J].1991,180(2).
- [10]Galbraith R.Heme oxygenase: who needs it?[J].1999,222(3).
- [11]Green CJ,Motterlini R,Vesely MJ,etc.Heme oxygenase-1 induction in skeletal muscle cells: hemin and sodium nitroprusside are regulators in vitro.[J].1998,275(4 Pt.1).
- [12]R Stocker,Y Yamamoto,AF McDonagh,etc.Bilirubin is an antioxidant of possible physiological importance[J].1987,235 (4792).