You Position: Home > Paper
Influence of interleukin-10 on nitric oxide /nitric oxide synthase system of the aorta
( views:128, downloads:0 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2001.06.005
- Key Word:
- 白细胞介素-10;一氧化氮;一氧化氮合酶
Abstract： 为探讨抗炎因子白细胞介素-10 (IL-10)对大鼠主动脉一氧化氮(NO)/一氧化氮合酶(NOS)系统的影响, 应用Griess试剂、～3H-瓜氨酸生成及蛋白免疫印迹杂交等方法, 测定IL-10孵育对血管NO释放、 NOS活性及表达的影响.结果发现细菌脂多糖(LPS)呈浓度依赖性地激活诱导型NOS (iNOS), 促进NO生成.IL-10 (10-10～10-8 g/ml)呈浓度依赖性地上调内皮型NOS (eNOS)蛋白表达及其活性, 但对iNOS活性及表达无明显影响.IL-10 (10-9～10-8 g/ml)显著抑制10 μg/ml LPS诱导的NO生成和iNOS激活; 而高浓度IL-10 (10-7 g/ml)则上调iNOS的活性, 对eNOS蛋白的表达和活性无明显影响.因此IL-10对NO/NOS系统具有双重影响, 一方面可抑制炎症介质诱发的作为炎性物质的iNOS的表达及激活, 另一方面可上调内皮源扩血管物质NO的释放.
- [1]Z, Mallat,C, Heymes,J, Ohan,etc.Expression of interleukin-10 in advanced human atherosclerotic plaques: relation to inducible nitric oxide synthase expression and cell death.[J].1999,19(3).
- [2]Z, Mallat,S, Besnard,M, Duriez,etc.Protective role of interleukin-10 in atherosclerosis.[J].1999,85(8).
- [3]Laurent Feldman,Luc Aguirre,Marianne Ziol,etc.Interleukin-10 Inhibits Intimal Hyperplasia After Angioplasty or Stent Implantation in Hypercholesterolemic Rabbits[J].2000,101(8).
- [4]Forstermann U,Li H.Nitric oxide in the pathogenesis of vascular disease.[J].2000,190(3).
- [5]L J, Pinderski Oslund,C C, Hedrick,T, Olvera,etc.Interleukin-10 blocks atherosclerotic events in vitro and in vivo.[J].1999,19(12).
- [6]Terkeltaub R. A..IL-10: An\"Immunologic Scalpel\" for Atherosclerosis?[J].1999.
- [7]R M, Clancy,A R, Amin,S B, Abramson.The role of nitric oxide in inflammation and immunity.[J].1998,41(7).