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Effect of interleukin-2 on intracellular calcium transients in rat ventricular myocytes
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.2001.06.004
- Key Word:
- 白介素-2;阿片受体;细胞内Ca2+;G蛋白;磷脂酶C
Abstract： 为研究白介素-2 (interleukin-2, IL-2)对心肌细胞内钙浓度([Ca2+]i) 的影响及其信号转导途径, 实验采用酶解法分离成年大鼠心室肌细胞, 以Fura-2/AM为钙探针, 用细胞内双波长钙荧光系统检测细胞[Ca2+]i的变化.结果发现: (1) IL-2 (0.5～200 U/ml)浓度依赖性地降低单个心室肌细胞内钙瞬态, IL-2 (200 U/ml) 对咖啡因诱导的肌浆网内储钙的释放无影响; (2) 纳洛酮(naloxone, Nal) (10-8 mol/L) 和nor-binaltorphimine (nor-BNI, 10-8 mol/L)可阻断IL-2对心肌细胞钙瞬态的作用, 而纳曲吲哚(naltrindole, NTI) (10-6 mol/L) 不能阻断此作用; (3) κ阿片受体激动剂U50488H (10-6 mol/L)降低心肌细胞钙瞬态, nor-BNI (10-8 mol/L)可阻断此作用; (4) 5 mg/L百日咳毒素(PTX)预处理可取消IL-2降低心肌细胞钙瞬态的作用, 而酪氨酸激酶抑制剂genistein (10-4 mol/L) 不能取消IL-2的作用; (5) U73122预处理可阻断IL-2的作用.研究结果表明, IL-2降低心肌细胞钙瞬态的作用, 是通过心肌细胞上κ阿片受体介导的, 其下游途径包括PTX敏感的G蛋白和磷脂酶C.
- [1]K.K. Tai,W-Q. Jin,T.K.Y. Chan,etc.Characterization of [3H]U69593 binding sites in the rat heart by receptor binding assays[J].1991,23(11).
- [2]D. Weisensee,J. Bereiter-Hahn,W. Schoeppe,etc.Effects of cytokines on the contractility of cultured cardiac myocytes[J].1993,15(5).
- [3]Kay, N.E.,Morley, J.E.,Allen, J.I..Interaction between endogenous opioids and IL-2 on PHA-stimulated human lymphocytes.[J].1990,70(4).
- [4]Jin-Song Bian,Wei-Min Zhang,Qiang Xia,etc.Phospholipase C Inhibitors Attenuate Arrhythmias Induced byκ-receptor Stimulation in the Isolated Rat Heart[J].1998,30(10).
- [5]T, Gulick,M K, Chung,S J, Pieper,etc.Interleukin 1 and tumor necrosis factor inhibit cardiac myocyte beta-adrenergic responsiveness.[J].1989,86(17).
- [6]Wu S.,Li H. Y.,Wong T. M..Cardioprotection of Preconditioning by Metabolic Inhibition in the Rat Ventricular Myocyte: Involvement of\u00a0-Opioid Receptor[J].1999.
- [7]Yoshitane Seino,Uichi Ikeda,Kenryo K. Minezaki,etc.Expression of cytokine-induced neutrophil chemoattractant in rat cardiac myocytes[J].1995,27(9).
- [8]Dorn GW.,Oral H.,Mann DL..Sphingosine Mediates the Immediate Negative Inotropic Effects of Tumor Necrosis Factor-Alpha in the Adult Mammalian Cardiac Myocyte[J].1997,272(8).
- [9]Xie, L.-H.,Horie, M.,James, A. F.,etc.Endothelin-1 inhibits L-type Ca currents enhanced by isoproterenol in guinea-pig ventricular myocytes[J].1996,431(4).
- [10]Wong T.M.,Sheng J.Z.,Wong N.S.,etc.Signal Transduction in the Cardiac k-Receptor[J].1995,4(3).
- [11]Salvatore Pepe,Rui-Ping Xiao,Charlene Hohl,etc.'Cross Talk' Between Opioid Peptide and Adrenergic Receptor Signaling in Isolated Rat Heart[J].1997,95(8).
- [12]B B, Farmer,M, Mancina,E S, Williams,etc.Isolation of calcium tolerant myocytes from adult rat hearts: review of the literature and description of a method.[J].1983,33(1).
- [13]Gesbert F,Delespine-Carmagnat M,Bertoglio J.Recent advances in the understanding of interleukin-2 signal transduction.[J].1998,18(5).
- [14]M S, Finkel,C V, Oddis,T D, Jacob,etc.Negative inotropic effects of cytokines on the heart mediated by nitric oxide.[J].1992,257.
- [15]C L, Jiang,D, Xu,Z D, You,etc.[Possible mechanism of the analgesic effect of interleukin-2 in central nervous system].[J].1996,48(3).