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5-HT3 receptors in amygdala mediate neuroimmunomodulation in rats
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- Author:
- No author available
- Journal Title:
- ACTA PHYSILOGOCA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0371-0874.2001.05.005
- Key Word:
- 杏仁核;脾细胞增殖;IL-2;5-HT3受体;神经免疫调制
Abstract： 实验通过大鼠侧脑室和杏仁核给予5-HT3受体激动剂1-phenylbiguanide (PBG), 用～3H-TdR掺入法测定脾细胞丝裂原(concanavalin A, Con A和lipopolysaccharide, LPS)刺激增殖效应, 用活化脾细胞增殖法测定IL-2生成, MTT法测定自然杀伤(natural killer, NK)细胞活性和用放射免疫测定血浆皮质酮水平, 以探讨大鼠杏仁核5-HT3受体在免疫调控中的作用.结果表明: 5-HT3受体拮抗剂granisetron (GNT, 0.1～0.4 mg/kg ip)剂量依赖地增强Con A和LPS刺激的脾细胞增殖, 作用在连续给药5 d最明显; 双侧脑室给予PBG (5 μg/side)可增强Con A和LPS刺激的脾细胞增殖效应, 作用在连续给药3 d最明显; 双侧和单侧中央杏仁核给予PBG 0.5 μg均增强Con A刺激的脾细胞增殖和IL-2生成; 底内侧杏仁核给予同剂量PBG仅增强LPS刺激的脾细胞增殖效应, 不影响Con A刺激的脾细胞增殖和IL-2生成; 中央杏仁核给予PBG升高血浆皮质酮的作用较底内侧杏仁核给予等量PBG引起的升高血浆皮质酮作用明显(P<0.01).侧脑室、中央杏仁核和底内杏仁核给予PBG对丝裂原刺激的脾细胞增殖效应影响不同, 但均被同时同部位给予GNT所拮抗, 提示杏仁核中央核和底内侧核的5-HT3受体可能以不同方式参与Con A或LPS刺激的脾细胞增殖效应的调制.
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