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Properties of KATP channels in hippocampal CA1 pyramidal neurons from adult rats
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- Author:
- No author available
- Journal Title:
- ACTA PHYSILOGOCA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0371-0874.2001.05.004
- Key Word:
- ATP敏感钾通道;海马;锥体神经元;膜片钳
Abstract： 为了解成年大鼠海马CA1区锥体细胞KATP通道的特性, 实验采用膜片钳技术的内面向外式记录法, 在急性分离的CA1区锥体神经元上, 研究了可被胞浆侧ATP所抑制的钾离子单通道的特性.当细胞膜内外两侧的K+浓度均为140 mmol/L时, 通道的电导为63 pS, 翻转电位为1.71 mV, 通道呈弱内向整流性.在负钳制电位时, 通道开放时常被短时程的关闭所打断, 而在正钳制电位时, 这种短时程的关闭状态明显少于负钳制电位时.但通道开放概率未见明显的电压依赖性.ATP对通道活动的抑制作用呈浓度依赖性, 抑制通道活动50%的ATP浓度为0.1 mmol/L.KATP通道的特异性阻断剂tolbutamide (甲糖宁, 1 mmol/L)可完全阻断通道的活动, 而KATP通道开放剂diazoxide (二氮嗪, 1 mmol/L)则不增强通道的活动.
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