Changes in the state of phosphorylation of DARPP-32 of gerbil striatum during ischemia

( views:57, downloads:0 )
SUN Ya-feng()
TANG Fang-Ming()
WANG Ran()
ZHANG Guang-Yi()
JIN Guo-Zhang()
Journal Title:
Volume 53, Issue 02, 2001
Key Word:

Abstract: Using the mongolian gerbil model of transient forebrain ischemia by bilateral carotid arteries occlusion, the levels of phosphorylation and the amount of DARPP-32 in striatum were measured by back-phosphorylation and Western-blotting assay in vitro. Transient forebrain ischemia had no effect on the immunoreaction of DARPP-32 in striatum, but the state of phosphorylation of DARPP-32 showed a significant change. The [32P] phosphate incorporation in DARPP-32 decreased after 2, 7 or 10 min of ischemia, but increased after a 5-min ischemia. In contrast, the result of back-phosphorylation in vivo was opposite to that obtained in vitro.

  • [1]Ren YB, Li XD, Xu ZC. Asymmetrical protection of neostriatal neurons against transient forebrain ischemia by unilateral dopamine depletion. Exp Neurology, 1997, 146:250~257.
  • [2]Hirotaka I, Mayumi O, Shigenobu S et al. Effect of transient forebrain ischemia on long-term enhancement of dopamine release in rat striatal slices. Brain Res, 1995, 671:95~99.
  • [3]Fienberg AA, Hiroi PG, Mermelstein WJ et al. DARPP-32: regulator of the efficacy of dopaminergic neurotransmission. Science, 1998, 281:838~842.
  • [4]Wozniak E, Oldziej S, Ciarkowski J. Molecular modeling of the catalytic domain of serine/threonine phosphatase-1 with the Zn2+ and Mn2+ nuclear ion centers in the active site. Comput Chem, 2000, 24:381~390.
  • [5]Toshiho O, Matsumoto M, Kitagawa K. Delayed neuronal death in ischemic hippocampus involves stimulation of protein tyrosine phosphorylation. Am J Physio, 1996, 271:1085~1097.
  • [6]Barone P, Morellim M, Dopoli M et al. Behavioural sensitization in 6-hydroxydopamined lesioned rats involves the dopamine signal transduction: changes in DARPP-32 phosphorylation. Neuroscience, 1994, 61:867~873.
  • [7]Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227:680~685.
  • [8]Hemmings HC, Greengard P. DARPP-32, a dopamine and adenosine 3′: 5′-monophosphate-regulated phosphoprotein: regional, tissue, and phylogenetic distribution. J Neurosci, 1986, 6(5):1469~1481.
  • [9]Kurihara T, Lewis RM, Eisler J et al. Cloning of cDNA for DARPP-32, a dopamine- and cyclic AMP-regulated neuronal phosphoprotein. J Neurosci, 1998, 8:508~517.
  • [10]Greengard P. Neuronal phosphoproteins. Mediators of signal transduction, Mol Neurobiol, 1987, 1:81~119.
  • [11]Snyder GL, Fienberg G, Dulubova I et al. Dopamine-mediated phosphorylation of NMDA-R1 in the rat nucleus accumbens. Society for Neuroscience Meeting, 1996, 22:380.
  • [12]Kegehr WQ, Tank DW. Calcium concentration dynamics produced by synaptic activation of CA1 hippocampal pyramidal cells. J Neurosci, 1992, 14:4202~4223.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615