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Substance P depresses GABA-activated currents in cultured hippocampal pyramidal neurons of rats

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Author:: XIONG Shun-Hua()
LI ZHI-WANG()
FAN You-Zhen()
Wang Ming-jiang()
WEI Jin-bo()

Journal Title:: ACTA PHYSIOLOGICA SINICA

Issue:: Volume 53, Issue 02, 2001

DOI:

Key Word:

Abstract： The purpose of the present study was to explore whether substance P (SP) modulates the response mediated by GABAA receptors. Experiments were carried out on cultured hippocampal pyramidal neurons of rats. GABA-activated inward currents were recorded using the whole-cell-patch-clamp techique. The majority of the neurons examined (66/92, 72%) were sensitive to both GABA and SP. When the neurons were treated with SP prior to application of GABA, the GABA-activated current (IGABA) was inhibited markedly, which was concentration-dependent and could be blocked by spantide, an NK1 receptor antagonist. With 10－8, 10－7, 10－6 and 10－5 mol/L SP, IGABA was inhibited by 18%, 24.8%, 25.9% and 28% respectively. Intracellular application of H7, a potent inhibitor of PKC, abolished inhibition of IGABA by SP, suggesting that the inhibition of IGABA by SP may be a result of intracellular phosphorylation of the GABAA receptor.

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