Correlation between enhanced anoxic tolerance induced by hypoxic preconditioning and the stability of mitochondrial membrane potential in cultured hypothalamic cells

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Author:
WU Li-ying()
DING Ai-shi()
MA Qiang()
WANG Fu-zhuang()
FAN Ming()
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 53, Issue 02, 2001
DOI:
Key Word:

Abstract: The relationship between enhanced anoxic tolerance induced by hypoxic preconditioning and mitochondrial membrane potential (MMP) was studied in cultured hypothalamic cells. Dynamic changes in MMP were monitored by confocal laser scanning microscopy and expression of B-cell lymphoma/leukemia-2 (bcl-2) was examined by flowcytometry. Hypoxic preconditioning increased the cell survival rate and decreased the lactate dehydrogenase leakage under acute anoxia, in addition to maintaining MMP at a relatively higher level and inducing bcl-2 overexpression during anoxia. The results suggest that hypoxic preconditioning can enhance the tolerance of hypothalamic cells to anoxia, and the underlying mechanism may be related to increased stability of MMP. Overexpression of bcl-2 induced by hypoxic preconditioning may play a role in sustaining the stability of MMP.

  • [1]Gorgias N, Maidatsi P, Tsolaki M et al. Hypoxic pretreatment protects against neuronal damage of the rat hippocampus induced by severe hypoxia. Brain Res, 1996, 714(1-2): 215~225.
  • [2]Ding AS (丁爱石), Wang FZ (王福庄), Wu LY(吴丽颖). Hypoxia preconditioning decreases apoptosis of cultured rat hippocampal neutrons after anoxia and reoxygenation. Chin J Neuroanat (神经解剖学杂志), 2000, 16(1): 7~10 (Chinese, English abstract).
  • [3]Smith DW, Buller KM, Day TA. Role of ventrolateral medulla catecholamine cells in hypothalamic neuroendocrine cell responses to systemic hypoxia. J Neurosci, 1995, 15(12): 7979~7988.
  • [4]Kelestimur H, Leach RM, Ward JP et al. Vasopressin and oxytocin release during prolonged environmental hypoxia in the rat. Thorax, 1997, 52(1): 84~88.
  • [5]Delcamp TJ, Dales C, Ralenkotter L et al. Intramitochondrial [Ca2+] and membrane potential in ventricular myocytes exposed to anoxia-reoxygenation. Am J Physiol, 1998, 275: H484~H494.
  • [6]Weinberg JM, Venkatachalam MA, Roeser NF et al. Mitochondrial dysfunction during hypoxia/reoxygenation and its correction by anaerobic metabolism of citric acid cycle intermediates. Proc Natl Acad Sci USA, 2000, 97: 2826~2831.
  • [7]Shen HM, Ong CN, Shi CY. Involvement of reactive oxygen species in aflatoxin B1-induced cell injury in cultured rat hepatocytes. Toxicology, 1995, 99(1-2):115~123.
  • [8]Gary EG, Pamela N, Lourdes TB. Synaptosomal plasma and mitochondrial membrane potentials during anoxia. Neurosci Lett, 1992, 138: 133~136.
  • [9]Ura H, Hirata K, Katsuramaki T. Mechanisms of cell death in hypoxic stress. Nippon Geka Gakkai Zasshi, 1999, 100(10): 656~662.
  • [10]Bernardi P, Scorrano L, Colonna R et al. Mitochondria and cell death. Mechanistic aspects and methodological issues. Eur J Biochem, 1999, 264(3): 687~701.
  • [11]Eyal Gottlieb, Matthew G, Vander Heiden et al. Bcl-XL prevents the initial decrease in mitochondrial membrane potential and subsequent reactive oxygen species production during tumor necrosis factor alpha-induced apoptosis. Mol Cell Biol, 2000, 20(15): 5680~5689.
  • [12]Marzo I, Bernner C, Kroemer G. The central role of the mitochondrial megachannel in apoptosis: evidence obtained with intact cells, isolated mitochondria, and purified protein complexes. Biomed Pharmacother, 1998, 52(6): 248~251.
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