Non-NMDA receptors are involved in the modulation of discharge activity of biphasic expiratory neurons and inspiratory neurons

( views:54, downloads:0 )
Author:
PAN Bing-xing()
WU Zhong-Hai()
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 53, Issue 02, 2001
DOI:
Key Word:

Abstract: Experiments were performed on in vitro brainstem slice preparations from neonatal rats that retain respiratory network activity. Extracellular recordings were made from 99 neuronal units, respiratory-related or non-respiratory related with rhythmical activity, out of which there were 7 biphasic expiratory and 11 inspiratory ones. Possible roles of non-NMDA receptors in reciprocal excitation among the biphasic expiratory neurons and in excitatory synaptic inputs to inspiratory neurons were investigated by administration of non-NMDA receptor agonist KA and its antagonist DNQX in the perfusion solution. Bath application of non-NMDA receptor agonist KA increased the peak frequency of both biphasic expiratory and inspiratory neuronal discharges, and increased the discharge frequency of the biphasic expiratory neurons and the inspiratory neurons in the middle phase, while the frequency of discharge in the early and late phases were less affected. All of these effects were blocked by addition of the non-NMDA receptor antagonist DNQX, suggesting the involvement of non-NMDA receptors.

  • [1]Collingridge CL, Lester BAJ. Excitatory amino acid receptors in the vertebrate central nervous system. Pharmacol Rev, 1989, 40(2):143~210.
  • [2]Honore T. Excitatory amino acid receptor subtypes and specific antagonists. Med Res Rev, 1989, 9:1~23.
  • [3]Westbrook GL, Jahr CE. Glutamate receptors in the excitatory neurotransmission. Seminars in the Neurosci, 1989, 1:103~114.
  • [4]Funk GD, Smith JC, Feldman JL. Generation and transmission of respiratory oscillation in medullary slices: role of excitatory amino acids. J Neurophysiol, 1993, 70:1497~1515.
  • [5]Greer JJ, Smith JC, Feldman JL. Roles of excitatory amino acids in the generation and transmission of respiratory drive in neonatal rat. J Physiol, 1991, 437:727~749.
  • [6]Liu G, Feldman JL, Smith JC. Excitatory amino acid-mediated transmission of inspiratory drive to phrenic motoneurons. J Neurophysiol, 1990, 64:423~436.
  • [7]Otsuka H, Lindah SGE, Lagercrantz H et al. Effects of NMDA and non-NMDA antagonists on respiratory neurons in the in vitro brainstem-spinal cord preparation of newborn rat. Neurosci Lett, 1994, 171:94~96.
  • [8]Onimaru H, Arata A, Homma I. Primary respiratory rhythm generator in the medulla of brainstem-spinal cord preparation from newborn rat. Brain Res, 1988, 445:314~324.
  • [9]Onimaru H, Arata A, Homma I. Firing properties of respiratory rhythm generating neurons in the absence of synaptic transmission in rat medulla in vitro. Exp Brain Res, 1989, 76:530~536.
  • [10]Onimaru H, Homma I, Iwatsuki K. Excitation of inspiratory neurons by preinspiratory neurons in rat medulla in vitro. Brain Res Bull, 1992, 29:879~882.
  • [11]Kashiwagi M, Onimaru H, Homma I. Correlation analysis of respiratory neuron activity in ventrolateral medulla of brainstem-spinal cord preparation isolated from newborn rat. Exp Brain Res, 1993, 95:277~290.
  • [12]Smith JC, Ellenberger H, Ballanyi K et al. Pre-Btzinger complex: a brainstem region that may generate respiratory rhythm in mammals. Science, 1991, 254:726~729.
  • [13]Smith JC, Greer JJ, Liu GS et al. Neural mechanisms generating respiratory pattern in mammalian brain stem-spinal cord in vitro. Spatiotemperal patterns of motor and medullary neuron activity. J Neurophysiol, 1990, 64:1149~1169.
  • [14]Wu ZH (吴中海), Zhang FT (张枫桐), Xu XY (徐小元). Discharge patterns of respiratory related neurons in the medial areas of nucleus retrofacialis of medulla. Acta Physiol Sin (生理学报), 1997, 49(4):389~394 (Chinese, English abstract).
  • [15]Reckling JC, Feldman JL. Pre-Btzinger complex and pacemaker neurons: Hypothesized site and kernal for respiratory rhythm generation. Annu Rev Physiol, 1998, 60:385~405.
  • [16]Ballanyi K, Onimaru H, Homma I. Respiratory network function in the isolated brainstem-spinal cord of newborn rats. Prog Neurobiol, 1999, 59:583~634.
  • [17]Koshiya N, Smith JC. Neuronal pacemaker for breathing visualized in vitro. Nature, 1999, 400:360~363.
  • [18]Krolo M, Stuch EA, Tonkovic-capin M et al. Differential roles of ionotropic glutamate receptors in canine medullary inspiratory neurons of the ventral respiratory group. J Neurophysiol, 1999, 82:60~68.
  • [19]Foutz AS, Champagnant J, Denavitt-saubie M. N-Methyl-D-aspartate (NMDA) receptors control respiratory off-switch in cat. Neurosci Lett, 1988, 87:221~226.
  • [20]Pierrefiche O, Foutz AS, Chmpagnat J et al. NMDA and non-NMDA receptors may play distinct roles in timing mechanisms and transmission in the feline network. J Physiol, 1994, 474:509~523.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn