Intermittent hypoxia exposure prevents mtDNA deletion and mitochondrial structure damage produced by ischemia/reperfusion injury

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ZHONG Ning()
ZHU Hai-Feng()
ZHOU Zhao-Nian()
Journal Title:
Volume 52, Issue 05, 2000
Key Word:
mitochondrial DNA;polymerase chain reaction;ischemia/reperfusion injury;intermittent hypoxia;ultrastructure

Abstract: In the present study, polymerase chain reaction (PCR) was conducted to determine mtDNA4834 deletion, and myocardial ultrastructure was visualized by electron microscope to see whether intermittent hypoxia (high altitude) adaptation exerts some action on mitochondria against ischemia/reperfusion injury. Myocardial ischemia/reperfusion in isolated perfused rat hearts induced severe damage to the ultrastructure of myocardial mitochondria and mtDNA4834 deletion down to 87.5% of normoxia rats. After the rats were exposed to intermittent hypoxia (5000 m;6 h/d for 28 d), the myocardial structure was well reserved and mtDNA4834 deletion dropped to 28.57% of control (P<0.05). It is suggested that intermittent hypoxia adaptation prevents mtDNA deletion, and preserves normal structure of mitochondria, which would be beneficial to the maintenance of normal mitochondrial function, and increases tolerance of myocardium against ischemia/reperfusion injury.

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