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Isolation of regulation genes related with liver regeneration by representational difference analysis
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.2000.04.003
- Key Word:
- 肝再生;表达性差异显示分析 (RDA);基因克隆
Abstract： 利用表达性差异显示分析 (RDA)技术, 研究大鼠2/3肝部分切除后1 h肝组织中基因的选择性表达, 建立了大鼠2/3肝部分切除术后1 h再生肝组织选择性表达基因EST库.该库约含3×104个独立克隆, 其中95%以上的克隆含有插入片段, 长度约200～700 bp不等, 对随机挑出的52个克隆的序列分析表明其中大多数基因与肝再生调控相关(38/52).10株未报道序列经RNA杂交证实, 其中6株与肝再生相关.
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