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Capacitating action of GABA and progesterone in spermatozoa of human and guinea pig in vitro
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0371-0874.2000.03.001
- Key Word:
- GABA;孕酮;获能;超激活运动;顶体反应;精子
Abstract： 为了探讨γ-氨基丁酸(GABA)是否参与人及豚鼠精子体外获能的调节, 将生育男子和豚鼠精子分别悬浮于BWW和低Ca2+最小获能培养基(LCa2+-MCM)中,加入GABA、孕酮(P4)、GABAA受体激动剂及其拮抗剂,在5% CO2孵箱38.5℃培养2 h.然后用ionophore A23187激发精子顶体反应(AR)和超激活运动(HAM).以精子与金霉素(CTC)荧光结合类型、AR和HAM为指标来评价精子获能情况.结果表明:(1)GABA和P4可明显地增加精子AR,这种作用可被GABAA受体激动剂muscimol所替代.GABA所致的获能作用不仅可被GABAA受体拮抗剂(-)荷包牡丹碱和印防己苦毒素所阻断,而且可被Ca2+螯合剂EGTA所抑制.(2)GABA具有明显地增强P4对精子的获能作用,并可明显地激发豚鼠精子的HAM.上述结果表明,GABA参与人及豚鼠精子体外获能的调节,这种作用通过精子表面的GABAA受体所介导.
- [1]袁玉英,张振汉,石其贤.卵丘细胞及胞外基质诱发中国仓鼠精子顶体反应[J].1998,(4).
- [2]袁玉英,何春娜,石其贤.γ-氨基丁酸诱发人精子顶体反应及其受精能力[J].1998,(3).
- [3]Aanesen, A.,Fried, G.,Anderssona, E.,etc.Evidence for gamma-aminobutyric acid specific binding sites on human spermatozoa[J].1995,10(7).
- [4]Q X, Shi,Y Y, Yuan,E R, Roldan.gamma-Aminobutyric acid (GABA) induces the acrosome reaction in human spermatozoa.[J].1997,3.
- [5]E R, Roldan,T, Murase,Q X, Shi.Exocytosis in spermatozoa in response to progesterone and zona pellucida.[J].1994,266(5190).
- [6]M A, de las Heras,A, Valcarcel,L J, Perez.In vitro capacitating effect of gamma-aminobutyric acid in ram spermatozoa.[J].1997,56.
- [7]M A, Lee,G S, Trucco,K B, Bechtol,etc.Capacitation and acrosome reactions in human spermatozoa monitored by a chlortetracycline fluorescence assay.[J].1987,48(4).
- [8]MURASE Tetsuma,ROLDAN Eduardo R. S..Progesterone and the zona pellucida activate different transducing pathways in the sequence of events leading to diacylglycerol generation during mouse sperm acrosomal exocytosis[J].1996.
- [9]王春年,刘海卫,袁玉英,等.γ-氨基丁酸诱发人精子顶体反应及其对若干离子转运影响的研究[J].1996,(2).
- [10]C A, Wistrom,S, Meizel.Evidence suggesting involvement of a unique human sperm steroid receptor/Cl- channel complex in the progesterone-initiated acrosome reaction.[J].1993,159(2).
- [11]贾振宇,袁玉英,石其贤.孕酮诱发豚鼠精子顶体反应过程中的Ca2+内流通道[J].1997,(3).
- [12]Valcarcel A,Heras MA de las,Perez LJ.The storage of pure ram semen at room temperature results in capacitation of a subpopulation of spermatozoa.[J].1997,47(2).
- [13]Q X, Shi,E R, Roldan.Evidence that a GABAA-like receptor is involved in progesterone-induced acrosomal exocytosis in mouse spermatozoa.[J].1995,52.
- [14]S. L. Erdö,B. Rosdy,L. Szporny.Higher GABA Concentrations in Fallopian Tube Than in Brain of the Rat[J].1982,38(4).