Effect of intracerebralventricular injection of nitric oxide donor and nitric oxide synthase inhibitor on the spontaneous discharge of magnocellular neurons of paraventricular nucleus in the rat

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Author:
JI Ya-Ping()
MEI Jun()
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 52, Issue 01, 2000
DOI:
Key Word:
nitric oxide;vasopressin;oxytocin;paraventricular nucleus

Abstract: The effects of intracerebralventricular (icv) injection of nitric oxide (NO) donor and nitric oxide synthase (NOS) inhibitor on the spontaneous discharge of magnocellular neurons of paraventricular nucleus (PVN) of hypothalamus were investigated in urethane anaesthetized adult SD rats by using microelectrode extracellularly recording method. The results showed that icv injection of NO donor sodium nitroprusside (SNP) had dose-dependent inhibitory effects on vasopressin (VP) secreting units in PVN. Icv injection of NOS inhibitor Nω-nitro-L-arginine methyl ester (L-NAME) also inhibited all of the VP-secreting neurons examined. Both the drugs had no effect on almost all oxytocin (OT) secreting units. These results suggest that NO may differentially modulate the activities of OT and VP neurons.

  • [1]Renaud LP, Bourque CW. Neurophysiology and neuropharmacology of hypothalamus magnocellular neurons secreting vasopressin and oxytocin. Prog Neurobiol, 1991, 36: 131~169.
  • [2]Schuman EM, Madison DV. Nitric oxide and synaptic func~tion. Annu Rev Neurosci, 1994, 17: 153~183.
  • [3]Bredt DS,Hwang PH,Snyder SH.Localization of nitric oxide synthase indicating a neural role for nitric oxide. Nature, 1990, 347: 768~770.
  • [4]Miyagawa A, Okamura H, Ibata Y. Coexistence of NADPH-diaphorase magnocellular neurons of the paraventricular and the supraoptic nuclei of the rat hypothalamus. Neurosci Lett, 1994, 176: 13~16.
  • [5]Sanchez F, Alonso JR, Arevalo R, et al. Coexistence of NADPH-diaphorase with vasopressin and oxytocin in the hypothalamic magnocelluar neurosecretory nuclei of the rat. Cell Tissue Res, 1994, 276: 31~34.
  • [6]Bains JS, Ferguson AV. Angiotensin neurotransmitter actions in paraventricular nucleus are potentiated by a nitric oxide synthase inhibitor. Regul Pept, 1994, 50: 53~59.
  • [7]Summy-Long JY, Bui V, Mantz S, et al. Central inhibition of nitric oxide synthase preferentially augments release of oxytocin during dehydration. Neurosci Lett, 1993, 152: 190~193.
  • [8]Ji YP (纪亚平), Mei J(梅俊), Tang B (唐斌). Effects of intracerebralventricular administration of norepineprine on paraventricular neurosecretory cells. Acta Physiol Sinica (生理学报), 1995, 47: 120~126 (in Chinese with English abstract).
  • [9]Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordi~nates, 2nd ed. Sydney: Academic Press, 1986.
  • [10]Yagi K, Azuma T, Matsuda K. Neurosecretory cell: capable of conducting impulse in rats. Science, 1966, 154: 778~779.
  • [11]Day TA, Ferguson AV, Renaud, LP. Facilitatory influence of noradrenergic afferents on the excitability of rat paraventricular nucleus neurosecretory cells. J Physiol, 1984, 355: 237~249.
  • [12]Horn T, Smith PM, Mclaughlin BE, et al. Nitric oxide actions in paraventricular nucleus: cardiovascular and neurochemical implications. Am J Physiol, 1994, 266: 306~313.
  • [13]Bains JS, Ferguson AV. Nitric oxide regulated NMDA driven GABA-ergic inputs to typeⅠneurons of the rat paraventricular nucleus. J Physiol (Lond), 1997, 499: 733~746.
  • [14]Cao L, Sun X, Shen E. Nitric oxide stimulates both the basal and reflex release of vasopressin in anaesthetized rats. Neurosci Lett, 1996, 221: 49~52.
  • [15]Okere CO, Wang YF, Higuchi T, et al. The effect of systemic and central nitric oxide administration on milk availability in lactating rats. NeuroReport, 1996, 8: 243~247.
  • [16]Shen E. Expression of immediate early gene and functions of nitric oxide in magnocellular neurosecretory neurons. Caps News Communi, 1996, 15: 12~16.
  • [17]Kadekaro M, Terrell ML, Liu H, et al. Effects of L-NAME on cerebral metabolic, vasopressin, oxytocin, and blood pressure responses in hemorrhaged rats. Am J Physiol, 1998, 274: R1070~R1077.
  • [18]Melaragno MG, Fink GD. Role of ANG-Ⅱ hypertension pro~duced by chronic inhibition of nitric oxide synthase in conscious rats. Am J Physiol, 1996, 271: H806~H811.
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