Effect of intracerebralventricular injection of nitric oxide donor and nitric oxide synthase inhibitor on the spontaneous discharge of magnocellular neurons of paraventricular nucleus in the rat
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.2000.01.007
- Key Word:
- 一氧化氮;加压素;催产素;室旁核;nitric oxide;vasopressin;oxytocin;paraventricular nucleus
Abstract： The effects of intracerebralventricular (icv) injection of nitric oxide (NO) donor and nitric oxide synthase (NOS) inhibitor on the spontaneous discharge of magnocellular neurons of paraventricular nucleus (PVN) of hypothalamus were investigated in urethane anaesthetized adult SD rats by using microelectrode extracellularly recording method. The results showed that icv injection of NO donor sodium nitroprusside (SNP) had dose-dependent inhibitory effects on vasopressin (VP) secreting units in PVN. Icv injection of NOS inhibitor Nω-nitro-L-arginine methyl ester (L-NAME) also inhibited all of the VP-secreting neurons examined. Both the drugs had no effect on almost all oxytocin (OT) secreting units. These results suggest that NO may differentially modulate the activities of OT and VP neurons.
- [1]Miyagawa, A.,Okamura, H.,Ibata, Y..Coexistence of oxytocin and NADPH-diaphorase in magnocellular neurons of the paraventricular and the supraoptic nuclei of the rat hypothalamus[J].1994,171(1).
- [2]L P, Renaud,C W, Bourque.Neurophysiology and neuropharmacology of hypothalamic magnocellular neurons secreting vasopressin and oxytocin.[J].1991,36(2).
- [3]Bains J S,Ferguson A V.Nitric oxide regulates NMDA-driven GABAergic inputs to type I neurones of the rat paraventricular nucleus.[J].1997,499(3).
- [4]Bains, J. S.,Ferguson, A. V..Angiotensin II neurotransmitter actions in paraventricular nucleus are potentiated by a nitric oxide synthase inhibitor[J].1994,50(1).
- [5]T, Horn,P M, Smith,B E, McLaughlin,etc.Nitric oxide actions in paraventricular nucleus: cardiovascular and neurochemical implications.[J].1994,266(1 Pt 2).
- [6]Day T A,Ferguson A V,Renaud L P.Facilitatory influence of noradrenergic afferents on the excitability of rat paraventricular nucleus neurosecretory cells.[J].1984,355(1).
- [7]D S, Bredt,P M, Hwang,S H, Snyder.Localization of nitric oxide synthase indicating a neural role for nitric oxide.[J].1990,347(6295).
- [8]Kinji Yagi,Takehiko Azuma,Kojiro Matsuda.Neurosecretory Cell: Capable of Conducting Impulse in Rats[J].1966,154(3750).
- [9]E M, Schuman,D V, Madison.Nitric oxide and synaptic function.[J].1994,17.
- [10]Chuma Okere,Yu-Feng Wang,Takashi Higuchi,etc.The effect of systemic and central nitric oxide administration on milk availability in lactating rats[J].1996,8(1).
- [11]F.Sánchez,J.R.Alonso,R.Arévalo,etc.Coexistence of NADPH-diaphorase with vasopressin and oxytocin in the hypothalamic magnocellular neurosecretory nuclei of the rat[J].1994,276(1).
- [12]Joan Y. Summy-Long,Vuong Bui,Shelley Mantz,etc.Central inhibition of nitric oxide synthase preferentially augments release of oxytocin during dehydration[J].1993,152(1-2).
- [13]Cao L,Shen E,Sun X.Nitric oxide stimulates both the basal and reflex release of vasopressin in anesthetized rats.[J].1996,221(1).