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MODULATION OF GLYCINE-ACTIVATED CHLORIDE CURRENTS BY SUBSTANCE P IN RAT SACRAL DORSAL COMMISSURAL NEURONS

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Author:
No author available
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
4
DOI:
10.3321/j.issn:0371-0874.1999.04.001
Key Word:
P物质;甘氨酸;骶髓后连合核;蛋白激酶C;钙离子/钙调素依赖性的蛋白激酶Ⅱ;substance P;glycine;sacral dorsal commissural nucleus;protein kinase C;Ca2+/calmodulin-dependent protein kinase Ⅱ

Abstract: The modulatory effect of substance P (SP) on strychnine-sensitive glycine (Gly) response was examined in neurons acutely dissociated from the rat sacral dorsal commissural nucleus (SDCN) using nystatin perforated patch recording configuration under voltage-clamp conditions. Application of SP potentiated 30 μmol/L Gly-activated chloride current (IGly) in a concentration-dependent manner over the range of 1 nmol/L to 1 μmol/L at a holding potential of -40 mV. SP neither changed the reversal potential of Gly response nor affected the affinity of Gly to its receptor. The SP potentiation effect could be blocked by spantide as well as a selective NK1 receptor antagonist, L-668,169, but not by NK2 receptor antagonist, L-659,877. The facilitatory action of SP on IGly could also be abolished by pretreatment with chelerythrine or KN-62 in different neurons, a finding suggesting that protein kinase C (PKC) or Ca2+/calmodulin-dependent protein kinase Ⅱ (CaMKⅡ) possibly contributes to an intracellular pathway of SP in the augmentation of IGly. The results imply that SP may suppress nociception in the spinal cord by potentiating Gly response.

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