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ET-1 INDUCES THE EXPRESSION OF PROONCOGENE c-fos IN CULTURED NEONATAL RAT MYOCARDIAL CELLS
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.1999.01.004
- Key Word:
- 内皮素-1;心肌细胞;c-fos
Abstract： 本实验用无血清的培养新生大鼠心肌细胞,探讨内皮素-1(ET-1)对原癌基因c-fos表达的作用.结果显示: ET-1可显著诱导c-fos的表达,其表达的高峰在30 min,2 h恢复到正常水平,并呈剂量依赖性反应和被ETA的特异性受体拮抗剂BQ123所阻断;蛋白激酶C(PKC)激动剂PMA可诱导c-fos表达,而PKC抑制剂Staurosporine则可阻断ET-1诱导的c-fos表达;钙通道阻断剂硝苯吡啶预处理心肌细胞对ET-1诱导的心肌细胞的c-fos表达无明显的作用.这些结果提示,ET-1诱导c-fos表达是通过ETA受体介导的,PKC在此过程中起重要作用.
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