You Position: Home > Paper
EFFECT OF HYPOXIA ON ANGIOTENSIN-CONVERTING ENZYME ACTIVITY AND GENE EXPRESSION OF INTRAPULMONARY ARTERY SMOOTH MUSCLE CELLS
( views:416, downloads:4 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.1998.06.004
- Key Word:
- 低氧;肺动脉;平滑肌细胞;血管紧张素转化酶(ACE);兔
Abstract： 实验采用分离培养兔肺内小动脉平滑细胞(PASMC),观察低氧对PASMC的血管紧张素转化酶(ACE)活性和基因表达的影响.结果表明:(1)正常情况下培养的兔PASMC中有ACE基因表达并可检测到其活性,而培养基中未检测到ACE活性;(2)低氧6 h后,ACE基因表达变化不明显,但在随后的12、24、48h后表达明显增加,呈时间依赖性;(3)低氧24h后,PASMC细胞中及条件培养基中ACE活性均显著增加.上述结果提示:(1)基础状态下培养的兔PASMC存在ACE;(2)低氧可促进PASMC ACE活性和基因表达,这可能与低氧性肺动脉高压有关.
- [1]R D, Farhy,O A, Carretero,K L, Ho,etc.Role of kinins and nitric oxide in the effects of angiotensin converting enzyme inhibitors on neointima formation.[J].1993,72.
- [2]Z, Nong,J M, Stassen,L, Moons,etc.Inhibition of tissue angiotensin-converting enzyme with quinapril reduces hypoxic pulmonary hypertension and pulmonary vascular remodeling.[J].1996,94.
- [3]Gibbons G H,Pratt R E,Dzau V J.Vascular smooth muscle cell hypertrophy vs. hyperplasia. Autocrine transforming growth factor-beta 1 expression determines growth response to angiotensin II.[J].1992.
- [4]N, Shiota,M, Miyazaki,H, Okunishi.Increase of angiotensin converting enzyme gene expression in the hypertensive aorta.[J].1992,20.
- [5]S, Oparil,A J, Narkates,R M, Jackson,etc.Altered angiotensin-converting enzyme in lung and extrapulmonary tissues of hypoxia-adapted rats.[J].1988,65.
- [6]Ikram H,Maslowski A H,Nicholls M G,etc.Haemodynamic and hormonal effects of captopril in primary pulmonary hypertension.[J].1982.
- [7]J C, Hartman,T M, Wall,T G, Hullinger,etc.Reduction of myocardial infarct size in rabbits by ramiprilat: reversal by the bradykinin antagonist HOE 140.[J].1993,21.
- [8]Rakugi H,Kim D K,Krieger J E,etc.Induction of angiotensin converting enzyme in the neointima after vascular injury. Possible role in restenosis.[J].1994.
- [9]S P, Janssens,B T, Thompson,C R, Spence,etc.Polycythemia and vascular remodeling in chronic hypoxic pulmonary hypertension in guinea pigs.[J].1991,71.
- [10]Schunkert H,Dzau V J,Tang S S,etc.Increased rat cardiac angiotensin converting enzyme activity and mRNA expression in pressure overload left ventricular hypertrophy. Effects on coronary resistance, contractility, and relaxation.[J].1990.
- [11]David G.Kiely,Robert I.Cargill,Brian J.Lipworth.Acute hypoxic pulmonary vasoconstriction in man is attenuated by type I angiotensin II receptor blockade[J].1995,30(6).
- [12]Brian J. Lipworth,Kenneth D. Dagg.Vasoconstrictor Effects of Angiotensin II on the Pulmonary Vascular Bed[J].1994,105(5).
- [13]B\u00f6nner G.,Preis S.,Schunk U.,etc.Effect of Bradykinin on Arteries and Veins in Systemic and Pulmonary Circulation[J].1992.
- [14]R B, Innis,D C, Manning,J M, Stewart,etc.[3H]Bradykinin receptor binding in mammalian tissue membranes.[J].1981,78.
- [15]Stenmark KR,Morris KG,Atochina EN,etc.Angiotensin converting enzyme expression is increased in small pulmonary arteries of rats with hypoxia-induced pulmonary hypertension.[J].1995,96(4).