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INVOLVEMENT OF ENDOGENOUS OPIOIDS IN CARDIOPROTECTIVE EFFECTS OF ISCHEMIC PRECONDITIONING IN THE ISOLATED RAT HEART
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0371-0874.1998.06.001
- Key Word:
- 阿片物质;缺血预处理;心律失常
Abstract： 实验以离体灌流的SD大鼠心脏为模型,用κ特异性拮抗剂MR2266研究κ阿片受体的阻断与缺血预处理(IP)的关系,用放射免疫分析法研究IP及长时间缺血对心肌强啡肽A1-13(Dyn A1-13)浓度的影响,探索κ阿片物质在IP过程中的作用和地位.结果显示:(1)IP可减轻缺血/复灌性心律失常严重程度(P＜0.05),缩小心肌梗死范围(P＜0.01),对冠脉流量和心率无明显影响;(2)MR2266可减轻缺血/复灌性心律失常严重程度(P＜0.05),缩小心肌梗死范围(P＜0.01),促进复灌过程中冠脉流量的恢复,对心率无明显影响;(3)空白灌流对照组心房肌Dyn Al-13浓度(pg/mg心肌湿重)为1.14±0.44,有IP的长时间缺血组和无IP的长时间缺血组心房肌Dyn A1-13浓度分别为0.56±0.23和0.41±0.14;对照组心室肌浓度为0.76±0.25,有IP的长时间缺血组和无IP的长时间缺血组心室肌DynA1-13浓度分别为0.49±0.24和0.28±0.09.可见缺血使Dyn A1-13浓度降低(P＜0.05),缺血前未经过IP处理的心脏这种降低较之经过IP处理的心脏更明显(P＜0.05).结果提示:(1)κ阿片受体拮抗剂MR2266能"模拟"IP的抗心律失常作用和缩小心肌梗死范围;(2)缺血可能引起κ阿片物质释放,经过IP处理的心肌在随后较长时间缺血时释放的内源性κ阿片物质减少;(3)IP的心肌保护过程涉及内源性κ阿片物质.
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