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NEUROKININ-1 RECEPTOR MEDIATED FORMALIN-INDUCED c-fos EXPRESSION IN THE RAT SPINAL CORD
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.1998.04.001
- Key Word:
- P物质;痛;c-fos;脊髓
Abstract： 本文用Fos作为背角伤害性反应神经元活动的一个标志物,结合免疫细胞化学和神经药理学方法,观察了速激肽受体拮抗剂对福尔马林诱发的脊髓c-fos原癌基因表达的影响.一侧大鼠后肢跖部皮下注射福尔马林,仅在同侧脊髓背角有c-fos表达.Fos阳性神经元最密集分布于Ⅰ层和Ⅱ层背侧的内侧部,中等量分布于Ⅳ层和Ⅴ层,少量定位于Ⅱ层腹侧部、Ⅲ、Ⅵ和Ⅹ层.若预先在一侧大鼠后肢跖部皮下注射福尔马林前,鞘内给予神经激肽-1(NK-1)受体拮抗剂L-668,196(0.1,1,10μg)可以产生剂量依赖性福尔马林诱发的背角Fos阳性细胞数量减少(P＜0.01),而注射NK-2受体拮抗剂L-649,877(10μg)和NK-3受体拮抗剂SR-142,801(10μg)对福尔马林诱发的背角c-fos表达没有影响(P＞0.05).上述结果表明NK-1受体介导着福尔马林诱发的背角c-fos原癌基因表达,强烈地提示NK-1受体在脊髓伤害性信息传递中起着重要作用.
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