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PROPERTIES OF THE GM-CSF-INDUCED OUTWARD K + CURRENT IN MURINE PERITONEAL EXUDATE MACROPHAGES
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.1998.02.005
- Key Word:
- 粒细胞巨噬细胞集落刺激因子;外向K+电流;巨噬细胞;膜片箝
Abstract： 以粒细胞巨噬细胞集落刺激因子(GM-CSF,16 ng/ml)长期(0.5～6 d)刺激小鼠腹腔渗出的巨噬细胞,采用全细胞膜片箝技术研究在GM-CSF刺激过程中细胞膜电流的变化,观察到一种GM-CSF诱导的瞬间失活的外向K+电流(记作IA),该电流在生理电压范围内可发生稳态失活,且当施加0.5 Hz的去极化脉冲刺激时其失活具有频率依赖性.该电流对胞外4-AP(3 mmol/L)高度敏感,胞内Ca2+浓度[Ca2+]i升高可抑制其幅度,环己酮亚胺(0.3μg/ml)作用细胞12 h可抑制其表达.值得注意的是这种K+电流的表达和相应电导的激活行为及动力学均随GM-CSF刺激时间长短而异.GM-CSF作用2 d,表达该电流的细胞数增至最大(大约55%),通道蛋白的密度亦达高峰,该电流激活的半值电导电位V1/2最低,为-27.55 mV,电流激活和失活迅速.随着GM-CSF作用时间的继续延长,表达该电流的细胞数下降,通道蛋白不断下调,电流激活曲线向正电位方向移动,电流激活和失活的时间过程减慢.这些结果表明,GM-CSF可诱导一种瞬间失活的外向K+电流(IA)的表达,提示该电流可能与GM-CSF激活的巨噬细胞的功能活性状态密切相关.
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