You Position: Home > Paper
EFFECT OF AVP(4-8) ADMINISTRATION ON Ca2+/CaM-DEPENDENT PROTEIN KINASE Ⅱ AUTOPHOSPHORYLATION IN RAT BRAIN
( views:155, downloads:9 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.1998.02.002
- Key Word:
- 加压素(4-8);ZDC(C)PR;鼠脑;Ca2+/CaM依赖的蛋白激酶Ⅱ;自身磷酸化;免疫印迹;免疫沉淀
Abstract： 大鼠皮下注射加压素(AVP)(4-8)1 h后,大脑皮层中Ca2+/CaM依赖的蛋白激酶Ⅱ(CaMKⅡ)自身磷酸化程度与对照组比较增高192%,P＜0.001;海马中增高40%,P＜0.05.CaMKⅡ的自身磷酸化程度依赖于Ca2+及CaM的浓度.在用抗CaMKⅡα单克隆抗体对给药1 h组样品和对照组样品进行免疫印迹检测时,发现皮下注射AVP(4-8)1 h后,大脑皮层中CaMKⅡa亚基的蛋白量没有明显差异.AVP(4-8)的拮抗剂ZDC(C)PR可以明显阻断AVP(4-8)的作用,表明AVP(4-8)刺激CaMKⅡ的活化是通过受体介导的信号转导过程.
- [1]AJ Silva,R Paylor,JM Wehner,etc.Impaired spatial learning in alpha-calcium-calmodulin kinase II mutant mice[J].1992,257 (5067).
- [2]S G, Miller,M B, Kennedy.Regulation of brain type II Ca2+/calmodulin-dependent protein kinase by autophosphorylation: a Ca2+-triggered molecular switch.[J].1986,44(6).
- [3]P, Greengard,F, Valtorta,A J, Czernik,etc.Synaptic vesicle phosphoproteins and regulation of synaptic function.[J].1993,259.
- [4]Isao Ito,Hiroyoshi Hidaka,Hiroyuki Sugiyama.Effects of KN-62, a specific inhibitor of calcium/calmodulin-dependent protein kinase II, on long-term potentiation in the rat hippocampus[J].1991,121(1-2).
- [5]X W, Rong,X F, Chen,Y C, Du.Potentiation of synaptic transmission by neuropeptide AVP4-8 (ZNC(C)PR) in rat hippocampal slices.[J].1993,4(9).
- [6]J E, Lisman,M A, Goldring.Feasibility of long-term storage of graded information by the Ca2+/calmodulin-dependent protein kinase molecules of the postsynaptic density.[J].1988,85.
- [7]H, Schulman,L L, Lou.Multifunctional Ca2+/calmodulin-dependent protein kinase: domain structure and regulation.[J].1989,14(2).
- [8]McGlade-McCulloh, Ellen,Yamamoto, Hideyuki.Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein...[J].1993,362(6421).
- [9]S G, Miller,M B, Kennedy.Distinct forebrain and cerebellar isozymes of type II Ca2+/calmodulin-dependent protein kinase associate differently with the postsynaptic density fraction.[J].1985,260(15).
- [10]R C, Malenka,J A, Kauer,D J, Perkel,etc.An essential role for postsynaptic calmodulin and protein kinase activity in long-term potentiation.[J].1989,340.
- [11]Malinow, R.,Schulman, H..Inhibition of postsynaptic PKC or CaMKII blocks induction but not expression of LTP.[J].1989,245(4920).
- [12]P T, Kelly,T L, McGuinness,P, Greengard.Evidence that the major postsynaptic density protein is a component of a Ca2+/calmodulin-dependent protein kinase.[J].1984,81.
- [13]Chao Lin,Ren-Yi Liu,Yu-Cang Du.Cysteinyl methyl ester of AVP(4-8), a potent agonist on the maintenance of passive avoidance in rats[J].1990,11(4).
- [14]Qiao LY,DU YC.Involvement of a putative G-protein-coupled receptor and a branching pathway in argipressin (4-8) signal transduction in rat hippocampus[J].1998,(1).