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MODULATION OF BRADYKININ ON ATP-ACTIVATED CURRENTS IN ISOLATED RAT DRG NEURONS
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.1998.01.012
- Key Word:
- 缓激肽;背根神经节;ATP-激活电流;调制
Abstract： 在新鲜分离大鼠背根神经节(DRG)的56个细胞标本上,应用全细胞膜片箝技术进行记录.胞外加缓激肽(BK,10-6～10-4mol/L)引起的DRG细胞膜反应结果如下:(1)71.4%的细胞为内向电流,其电流反应的幅值具有明显的浓度依赖性;(2)12.5%的细胞为外向电流;(3)16.1%的细胞未引起可检测的膜反应.单独给予ATP(10-6～10-3mol/L)在大多数受检细胞(54/56)引起一浓度依赖性内向电流,并有明显的去敏感现象.预加BK 30 s后再加ATP,则ATP激活电流明显增强,其电流幅值的增强作用依赖于BK及ATP浓度.BK对ATP激活电流的增强作用以增强其峰电流为主,对稳态部分增强不明显.实验观察了BK增强作用的时程,在检测的4例细胞中发现BK均于预加30 s后起效,作用持续20 min以上.本文结果提示:BK在初级感觉神经元水平对ATP引起的兴奋性反应可能起到一种易化作用.
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