PROLACTIN INHIBITION OF FSH-INDUCED TISSUE TYPE PLASMINOGEN ACTIVATOR EXPRESSION IN CULTURED RAT GRANULOSA CELLS

( views:58, downloads:0 )
Author:
LIU YI-XUN()
LIU HAI-ZHEN()
CHEN Yi-jun()
TOR NY()
Journal Title:
ACTA PHYSIOLOGICA SINICA
Issue:
Volume 50, Issue 01, 1998
DOI:
Key Word:
prolactin;granulosa cells;plasminogen activator inhibitor type-Ⅰ;tissue type plasminogen activator;follicle stimulating hormone

Abstract: This study was designed to investigate whether prolactin (PRL)affects coordinated regulation of tissue plasminogen activator (tPA) and plasminogen activator inhibitor type-Ⅰ (PAI-Ⅰ) gene expression in rat granulosa cells in vitro. Several methods, such as SDS-PAGE, immunoblot etc. were used to detect the effect of PRL on tPA and PAI-Ⅰ gene expression. The results demonstrated: (1) PRL increases PAI-Ⅰ mRNA production in cultured granulosa cells. Inclusion of follicle stimulating hormone (FSH) with PRL has a synergistic effect on the increase of PAI-Ⅰ mRNA levels. After 48 h culture in the presence of FSH with PRL, a 7.8-fold increase in PAI-Ⅰ mRNA levels is observed as compared with PRL alone. The synergistic increase in PAI-Ⅰ mRNA levels occurs in a dose- and time-dependent manner; (2) the increase in PAI-ⅠmRNA synthesis in the cells by PRL alone, or PRL in combination with FSH, is well correlated with the changes of PAI-Ⅰ activity levels in the conditioned media; (3) PRL in culture also decreases FSH-induced tPA activity level in a dose-dependent fashion. The decrease in FSH-induced tPA activity level by PRL is correlated with an increase in the amount of PA-PAI-Ⅰ complexes in the conditioned media. This suggests that the decline of tPA activity is related to neutralization of tPA by the increased PAI-Ⅰ activity in the media.

  • [1]McNeilly AS. Prolactin and the control of gonadotropin secretion. J Endocrinol. 1987, 115:1 ~ 5
  • [2]Kawagoe S, Hiroi M. Involvement of prolactin in the control of follicular maturation. Gynecol Obstet Invest.1990, 29:51 ~ 55
  • [3]Dorrington JH, Core-Langton RF. Antigonadal action of prolactin: Further studies on the mechanism of inhibition of follicle-stimulating hormone-induced aromatase activity in rat granulosa cell cultures. Endocrinology, 1982,110:1701
  • [4]Hu ZY, Liu YX. Effect of prolactin on gonadotropin-induced ovarian estrogen and progesterone production in mouse. Acta Physiol Sin, 1995.47: 96~ 99, (in Chinese with English abstract)
  • [5]McNatty KP, Sawer RS, McNeilly AS. A possible role for prolactin in control of steroid secretion by the human Graafian follicle. Nature, 1974, 250:653 ~ 655
  • [6]Hu ZY, Liu YX. Prolactin inhibits gonado tropin-induced plasminogen activator activity and ovulation in mice.Acta Physiol Sin, 1993, 45:510 ~ 516, (in Chinese with English abstract)
  • [7]Veldhuis JD, Klase P, Hammond JM. Effect of prolactin upon steroidgenesis by porcine granulosa cells in vitro:Influence of cyto-differentiation. Endocrinology, 1980, 107: 42~46
  • [8]Beers WH, Strickland S, Reich E. Ovarian plasminogen activator: relationships to ovulation and hormonal regulation. Cell, 1975, 6:387
  • [9]Liu YX, Cajander SB, Ny T, et al. Gonadotropin regulation of tissue type and urokinase type plasminogen activators in rat granulosa and theca-interstitial cells during the periovulatory period. Mol Cell Endocrinol, 1987,54:221 ~ 229
  • [10]Hamada Y, Schlaff S, Kobayashi Y, et al. Inhibitory effect of prolactin on ovulation in the in vitro perfusedrabbit ovary. Nature, 1980, 285: 161
  • [11]Yoshimura Y, Maruyama K, Shiraki M, et al. Prolactin inhibits plasminogen activator activity in preovulatory follicles. Endocrinology, 1990, 126:631~636
  • [12]Ny T, Bjersing L, Hsueh AJW, et al. Cultured granulosa cells produce two plasminogen activators and an antiactivator, each regulated differently by gonadotropins. Endocrinology, 1985, 116: 1666~ 1668
  • [13]Liu YX. Interaction and regulation of plasminogen activators and their inhibitor in rat periovular follicles during periovulatory periods. Sci Sin, 1988, 31:47 ~ 57
  • [14]Liu YX, Peng XR, Ny T. Tissue-specific and time-coordinated hormone regulation of plasminogen activator inhibitor type-Ⅰ and tissue-type plasminogen activator in the rat ovary during gonadotropin-induced ovulation.Europ J Biochem, 1991, 195: 549~ 555
  • [15]Liu YX, Liu K, Peng XR, et al. Coordinated regulation of tissue type plasminogen activator and plasminogen activator inhibitor type-Ⅰ gene expression in hypophysectomized rat ovaries during GnRHa-induced ovulation. Sci Sin (B), 1994, 37:820~830
  • [16]Liu YX, Hsueh AJW. Synergism between granulosa and theca interstitial cells in estrogen biosynthesis by gonadotropin treated rat ovaries: Studies on the two cell, two-gonadotropin hypothesis using steroid antisera. Biol Reprod, 1986, 35: 27~36
  • [17]Zeheb R, G eehrter TD. Cloning and sequencing of cDNA for rat plasminogen activator inhibitor-Ⅰ. Gene, 1988,73:459~468
  • [18]Ny T, Leonardsson G, Hsueh AJW. Cloning and characterization of a cDNA for rat tissue-type plasminogen activator. DNA, 1988, 7:671 ~ 677
  • [19]Sokal RR, Roulf FJ. Comparisons among means. In: Biometry, the Princioals and Practice Statistics in Biological Research, 2nd ed. New York: WH Freeman & Co., 1981, 232~ 261
  • [20]Liu YX, Peng XR, Liu K, et al. Hormonal regulation of expression of tissue type plasminogen activator and plasminogen activator inhibitor type-Ⅰ in cultured rat granulosa cells. Science ( China), 1994, 37:271 ~ 279
  • [21]Loskutoff DJ, Sawdey M, Mimuro J. Type-Ⅰ plasminogen activator inhibitor. In: Coller BS ed. Progress in Hemostasis and Thrombosis. Philadelphia: Saunders, 1988, 87~ 115
  • [22]Jones PBC, Muse KN, Wilson EA, et al. Expression of plasminogen activator (PA) and a PA inhibitor in human granulosa cells from preovulatory follicles. J Clin Endocrinol Metab, 1988, 67:857
  • [23]Liu YX, Feng P, Zou RJ. Gonadotropin regulation of tissue type and urokinase-type plasminogen activator and plasminogen activator inhibitor type-Ⅰ in Rhesus monkey ovaries during periovulatory period. BiolReprod, 1992,46 (Suppl. 1), 456
  • [24]Politis I, Srikandakumar A, Turner JD, et al. Changes in and partial identification ofthe plasminogen activator and plasminogen activator inhibitor systems during ovarian follicular maturation in the pig. Biol Reprod, 1990,43:636 ~ 642
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn