You Position: Home > Paper
EFFECTS OF HYPOXIA ON MYOGLOBIN IN CULTURED RAT MYOCYTES
( views:15, downloads:0 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0371-0874.1997.05.004
- Key Word:
- 低氧;复氧;培养心肌细胞;肌红蛋白;环磷酸腺苷;心肌细胞收缩
Abstract： 本实验观察了低氧、复氧时培养的乳鼠心肌细胞肌红蛋白(Mb)、cAMP、心肌收缩频率的变化以及磷酸二酯酶抑制剂茶碱和抑制肌质网上钙释放的普鲁卡因对低氧下心肌细胞Mb表达和心肌细胞收缩频率的影响.结果表明,随低氧时间的延长Mb增加,cAMP和心肌收缩下降,Mb、cAMP和心肌细胞收缩频率经复氧可以得到恢复.普鲁卡因使低氧时心肌细胞的收缩频率更慢和Mb的表达减弱;茶碱使低氧下心肌细胞的收缩频率和Mb的表达增加.实验结果提示:低氧时Mb表达增加、cAMP和心肌收缩频率下降可能是对低氧的适应性改变,而Mb表达可能由胞内钙与cAMP参与.
- [1]DJ Livingston,GN La Mar,WD Brown.Myoglobin diffusion in bovine heart muscle[J].1983,220 (4592).
- [2]Y, Park,D K, Bowles,J P, Kehrer.Protection against hypoxic injury in isolated-perfused rat heart by ruthenium red.[J].1990,253(2).
- [3]E A, Braunlin,G M, Wahler,C R, Swayze,etc.Myoglobin facilitated oxygen diffusion maintains mechanical function of mammalian cardiac muscle.[J].1986,20.
- [4]Keith A. Webster,Nanette H. Bishopric.Molecular regulation of cardiac myocyte adaptations to chronic hypoxia[J].1992,24(7).
- [5]D J, Taylor,P M, Matthews,G K, Radda.Myoglobin-dependent oxidative metabolism in the hypoxic rat heart.[J].1986,63(3).
- [6]V, Riveros-Moreno,J B, Wittenberg.The self-diffusion coefficients of myoglobin and hemoglobin in concentrated solutions.[J].1972,247.
- [7]Kakei M,Noma A,Shibasaki T.Properties of adenosine-triphosphate-regulated potassium channels in guinea-pig ventricular cells.[J].1985,363(1).
- [8]Wilfrid B. Veldkamp,Joseph R. Votano.Effects of intermolecular interaction on protein diffusion in solution[J].1976,80(25).
- [9]E, Wiener,A, Scarpa.Activation of protein kinase C modulates the adenylate cyclase effector system of B-lymphocytes.[J].1989,264(8).
- [10]H C, Lee,W T, Clusin.Cytosolic calcium staircase in cultured myocardial cells.[J].1987,61.
- [11]G.Brum,V.Flockerzi,F.Hofmann,etc.Injection of catalytic subunit of cAMP-dependent protein kinase into isolated cardiac myocytes[J].1983,398(2).
- [12]R P, Cole.Skeletal muscle function in hypoxia: effect of alteration of intracellular myoglobin.[J].1983,53(1).
- [13]J B, Wittenberg.Myoglobin-facilitated oxygen diffusion: role of myoglobin in oxygen entry into muscle.[J].1970,50.
- [14]G, Callewaert,L, Cleemann,M, Morad.Epinephrine enhances Ca2+ current-regulated Ca2+ release and Ca2+ reuptake in rat ventricular myocytes.[J].1988,85.
- [15]S, Takeo,M, Sakanashi.Possible mechanisms for reoxygenation-induced recovery of myocardial high-energy phosphates after hypoxia.[J].1983,15(9).