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EFFECTS OF NO PRECURSOR AND DONOR ON NEURONAL ACTIVITY OF RAT HIPPOCAMPAL SLICES
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.1997.04.003
- Key Word:
- 一氧化氮(NO);海马脑片;亚甲基蓝
Abstract： 应用细胞外记录单位放电技术,在大鼠海马脑片上观察了左旋精氨酸(L-arg)、N-硝基左旋精氨酸(L-NNA)、SIN-1、及亚甲基蓝(methylene blue,MB)对CA1区神经元自发放电的影响,旨在了解左旋精氨酸:NO通路在海马放电中的作用及其可能的机制.实验结果如下:(1)用Larg(1 mmol/L)灌流海马脑片2 min,在54个放电单位中有42个单位(77.8%)放电频率降低,12个单位(22.2%)无明显反应.用L-NNA(0.15 mmol/L)灌流海马脑片2 min,在29个放电单位中有25个单位(86.2%)放电频率增加,4个单位(13.8%)无明显变化.L-NNA的作用可被预先灌流L-Arg而逆转.(2)给予一氧化氮(NO)供体SIN-1(5 mmol/L),25个单位(100%)放电频率均明显减少,呈明显的剂量依赖性.(3)给脑片灌流鸟苷酸环化酶抑制剂亚甲基蓝(3μmol/L)30 min后,10个单位的平均放电频率较对照时明显增加,但亚甲基蓝不能消除L-arg对海马神经元的作用.综合上述结果,似可以认为,静息的海马有基础性NO释放,NO可抑制海马的放电活动,其作用似不通过鸟苷酸环化酶介导.
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