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AN ANTIBODY RECOGNIZING NEURON SPECIFIC TUBULIN
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0371-0874.1997.04.001
- Key Word:
- 神经元;微管蛋白;抗肽抗体;翻译后加工
Abstract： 通过人工合成大鼠巢蛋白(Nestin)C-末端的一段20肽,制备得到了抗巢蛋白的抗体--Anti-Nes-2.我们发现该抗体除了能识别小鼠240 kD的巢蛋白条带外,还特异性识别另一分子量约为50 kD的蛋白条带.蛋白印迹反应提示,该50 kD蛋白可能与神经细胞的增殖和分化相关.经分离纯化和N-端氨基酸序列测定证实:该50 kD蛋白为微管蛋白(α-tubulin).我们发现,通过免疫吸附反应该抗体只能被脑中分离的微管蛋白所特异性吸附,而不能被肺来源的微管蛋白所吸附.蛋白印迹反应结果还表明:与一般的α-和β-tubulin在各种组织和细胞株中都有表达的情况不同,该50 kD蛋白只在小鼠发育不同时期的脑组织及神经前体细胞中特异性表达,并随神经发育过程其表达量不断增加;而在其它组织和所考察的细胞株中都不表达.鉴于以上结果,我们认为Anti-Nes-2抗体所识别的50 kD蛋白是神经细胞特异的α-tubulin,并可能是一种神经元特异的α-tubulin翻译后加工产物.
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