You Position: Home > Paper
EFFECT OF CAFFEINE AND THEOPHYLLINE ON Ca2+ SENSITIVITY OF TROPONIN AND Ca2+ RELEASE FROM SR IN SAPONIN-SKINNED RAT CARDIAC MUSCLE
( views:31, downloads:0 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0371-0874.1997.03.005
- Key Word:
- 皂素;肌钙蛋白Ca2+敏感性;肌浆网Ca2+释放;蜕膜心肌;咖啡因;茶碱;大鼠
Abstract： 在低浓度皂素(50 μg/ml)制备的浆膜蜕变(通透性增高)而肌浆网(SR)膜无损的蜕膜心肌标本上,以其收缩的幅度作为SR Ca2+释放的半定量指标;高浓度皂素(500μg/ml)制备的浆膜和SR膜均蜕变的蜕膜心肌标本,以其张力-pCa关系曲线以及产生50%最大张力所需的Ca2+浓度(pCa50)分别作为肌钙蛋白(TN)Ca2+敏感性的定性和定量指标.结果观察到:(1)在低浓度皂素蜕膜心肌标本上,5和10 mmol/L咖啡因分别引起约89.2±12.7和142.5±17 mg(n=4,P＜0.05)张力的强直收缩,而5 mmol/L茶碱未能引起明显的强直收缩;(2)在高浓度皂素蜕膜心肌标本上,5和10 mmol/L咖啡因及5 mmol/L茶碱均使张力-pCa关系曲线左移;pCa50较对照分别增加了0.261,0.274和0.212pCa单位(P均小于0.001).上述结果提示:咖啡因和茶碱均能增高TNCa2+敏感性;此外,咖啡因尚有促使SR释放Ca2+的作用.
- [1]M, B?hm,I, Morano,B, Pieske,etc.Contribution of cAMP-phosphodiesterase inhibition and sensitization of the contractile proteins for calcium to the inotropic effect of pimobendan in the failing human myocardium.[J].1991,68(3).
- [2]江明性.正性肌力作用的方式及新药[J].1991,(1).
- [3]陈松海,韩启德.细胞内游离CA（2+）浓度的调节机制[J].1993,(1).
- [4]I.R.Wendt,D.G.Stephenson.Effects of caffeine on Ca-activated force production in skinned cardiac and skeletal muscle fibres of the rat[J].1983,398(3).
- [5]黄焰.大鼠皂素蜕膜心肌肌钙蛋白Ca2+敏感性的实验研究[J].1996,(1).
- [6]M, Iino.Calcium-induced calcium release mechanism in guinea pig taenia caeci.[J].1989,94.
- [7]P M, Best,S K, Donaldson,W G, Kerrick.Tension in mechanically disrupted mammalian cardiac cells: effects of magnesium adenosine triphosphate.[J].1977,265(1).
- [8]J R, Blinks,C B, Olson,B R, Jewell,etc.Influence of caffeine and other methylxanthines on mechanical properties of isolated mammalian heart muscle. Evidence for a dual mechanism of action.[J].1972,30.
- [9]Bernd Wetzel,Norbert Hauel.New cardiotonic agents - a promising approach for treatment of heart failure[J].1988,9(5).
- [10]M.Endo,M.Iino.Specific perforation of muscle cell membranes with preserved SR functions by saponin treatment[J].1980,1(1).
- [11]G B, McClellan,S, Winegrad.The regulation of the calcium sensitivity of the contractile system in mammalian cardiac muscle.[J].1978,72.
- [12]Konishi M,Kurihara S,Sakai T.The effects of caffeine on tension development and intracellular calcium transients in rat ventricular muscle.[J].1984,355(1).