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STUDIES ON CHARACTERISTICS OF PROLIFERATION AND DIFFERENTIATION OF HEMATOPOIETIC STEM CELLS IN MICE
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0371-0874.1997.03.003
- Key Word:
- 造血干细胞;CFC-S;CFU-S;纤维母细胞;性别决定基因;PCR
Abstract： 本文选择Y染色体特异的性别决定基因(Sry)作为新的细胞遗传标志,采用PCR技术研究了小鼠造血干细胞的增殖与分化性能.将雄鼠骨髓细胞输注给经致死剂量射线照射的雌性受体小鼠,PCR测试结果表明,所有CFU-S均为供体起源.供体来源的CFU-S在其输入体内后,可通过增殖、分化形成各系造血细胞;但CFU-S中的纤维母细胞和CFU-S重建造血后受体小鼠骨髓中的纤维母细胞均为受体起源.由此可见,小鼠骨髓中的CFC-S是一类造血干细胞,它不具有分化为纤维母细胞的性能.
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