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2 HZ AND 100 HZ ELECTROACUPUNCTURE ACCELERATE THE EXPRESSION OF GENES ENCODING THREE OPIOID PEPTIDES IN THE RAT BRAIN
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- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0371-0874.1997.02.001
- Key Word:
- 电针刺激;刺激频率;前脑啡肽原;前强啡肽原;前阿黑皮素原;基因表达
Abstract： 我室以往的工作证明2 Hz和100 Hz电针可引起中枢释放不同种类的阿片肽,本工作试图阐明不同频率的电针是否影响三种阿片肽的基因转录.用地高辛标记的反义cRNA探针进行原位杂交,显示大鼠脑内前脑啡肽原(preproenkephalin,PPE),前强啡肽原(preprodynorphin,PPD)和前阿黑皮素原(proopiomelanocortin,POMC)mRNA.结果:(1)低、高频电针均不影响POMCmRNA的水平.(2)对PPE的影响,两种频率电针诱导脑干网状结构头端腹内侧区PPE mRNA升高的幅度相似,2 Hz电针诱导视上核、视交叉上核、下丘脑室旁核、弓状核、腹内侧核及外侧丘系核的PPE mRNA表达比100 Hz电针高得多.(3)对PPD的表达,2 Hz电针不改变脑内PPDmRNA的水平,100 Hz电针可使视上核、下丘脑室旁核、腹内侧核及脑干臂旁核的PPD mRNA明显升高.鉴于肽类的加速释放必然引起合成的增加,上述结果为不同频率电针加速不同内源性阿片肽的释放和合成,从而发挥镇痛作用,提供了有力的佐证.
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