You Position: Home > Paper
STUDIES ON DESENSITIZATION OF GABAB RECEPTOR COUPLED ADENYLATE CYCLASE
( views:77, downloads:0 )
- Author:
- No author available
- Journal Title:
- ACTA PHYSIOLOGICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0371-0874.1997.01.005
- Key Word:
- GABAB受体;腺苷酸环化酶;脱敏;蛋白激酶C
Abstract： 将突触体膜与佛波酯(PMA),GABAB受体激动剂巴氯芬(Baclofen,BAL)预孵育一定时间后,BAL对腺苷酸环化酶(AC)基础活性及forskolin刺激的AC活性的抑制率显著降低(脱敏);而forskolin预孵育时,BAL对基础及forskolin刺激的AC活性的抑制率不变,表明GABAB受体与AC偶联环节的脱敏机制涉及蛋白激酶C(PKC)激活,而与蛋白激酶A无关,脱敏时GABAB受体的Kd值增加.本实验提示,可能由于PKC激活导致GABAB受体结构或构象改变,使受体-G蛋白脱偶联而出现脱敏现象.
- [1]D R, Sibley,J L, Benovic,M G, Caron,etc.Regulation of transmembrane signaling by receptor phosphorylation.[J].1987,48(6).
- [2]Bowery, N.G.,Hill, D.R.,Hudson, A.L..Characteristics of GABA[J].1983,78(1).
- [3]O H, LOWRY,N J, ROSEBROUGH,A L, FARR,etc.Protein measurement with the Folin phenol reagent.[J].1951,193.
- [4]S.J. Enna,E.W. Karbon.GABAB receptors and transmitter-stimulated cAMP accumulation in rat brain[J].1984,23(Part 2).
- [5]L, Hahner,S, McQuilkin,R A, Harris.Cerebellar GABAB receptors modulate function of GABAA receptors.[J].1991,5(10).
- [6]Daniel J. Kelleher,Jeffrey E. Pessin,Arnold E. Ruoho,etc.Phorbol Ester Induces Desensitization of Adenylate Cyclase and Phosphorylation of the β -adrenergic Receptor in Turkey Erythrocytes[J].1984,81(14).
- [7]K, Taniyama,M, Niwa,Y, Kataoka,etc.Activation of protein kinase C suppresses the gamma-aminobutyric acidB receptor-mediated inhibition of the vesicular release of noradrenaline and acetylcholine.[J].1992,58.
- [8]G L, Kamatchi,M K, Ticku.GABAB receptor activation inhibits Ca2(+)-activated 86Rb-efflux in cultured spinal cord neurons via G-protein mechanism.[J].1990,506(2).