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Safety and effect of umbilical cord blood-derived mesenchymal stem cells on apoptosis of human cardiomyocytes

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Author:
No author available
Journal Title:
JOURNAL OF GERIATRIC CARDIOLOGY
Issue:
2
DOI:
No doi available
Key Word:
Umbilical cord blood;mesenchymal stem cells;5-azaserine;human cardiac myocyte;apoptosis

Abstract: Objective To study the safety and effect of the umbilical cord blood(UCB)-derived mesenchymal stem cells(MSCs)on apoptosis of human cardiomyocytes(HCM). Methods UCB was collected at the time of delivery with informed consent obtained from 10donors.The UCB-derived MSCs were treated with 5-azaserube(5-AZA)and were further induced to differentiate into cardiomyocytes.Telomerase activity,G-banding patterns of chromosomal karyotypes,tumor formation in nude mice,RT-PCR,and the effect of inhibiting apoptosis of HCM were investigated. Results MSCs derived from UCB were differentiated into cardiomyocytes in vitro,which possessed telomerase activity after 5-AZA induction,and no abnormal chromosomal karyotypes were observed.Expression of p53,cyclin A,cdk2,β-actin,C-fos,h-TERT and c-myc were similar in MSCs before and after 5-AZA treatment.There was no tumor formation in nude mice after injection of UCB-derived MSCs.UCB-derived MSCs significantly inhibited apoptosis of HCM. Conclusion UCB-derived MSCs are a valuable,safe and effective source of cell-transplantation treatment.

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