Hydrogen can alleviate post-cardiac arrest brain injury in rabbits

( views:226, downloads:0 )
Author:
WANG Jin-gao(Department of Emergency,the First Affiliated Hospital of Xiamen University,Xiamen, 361003, China)
LIN Ji-yan(Department of Emergency,the First Affiliated Hospital of Xiamen University,Xiamen, 361003, China)
KONG De-quan(Department of Emergency,the First Affiliated Hospital of Xiamen University,Xiamen, 361003, China)
ZHANG Zi-li(Department of Emergency,the First Affiliated Hospital of Xiamen University,Xiamen, 361003, China)
YU Xiang-mei(Department of Emergency,the First Affiliated Hospital of Xiamen University,Xiamen, 361003, China)
LIU Jia-quan(Department of Emergency,the First Affiliated Hospital of Xiamen University,Xiamen, 361003, China)
CAI Dong-mei(Department of Emergency,the First Affiliated Hospital of Xiamen University,Xiamen, 361003, China)
Journal Title:
Chinese Journal of Emergency Medicine
Issue:
Volume 21, Issue 10, 2012
DOI:
10.3760/cma.j.issn.1671-0282.2012.10.010
Key Word:
Cardiopulmonary resuscitation; Systemic inflammatory response syndrome; Post-cardiac arrest syndrome; Tumor necrosis factor-α; Neurological deficit scores.

Abstract: Objective To investigate the effects of hydrogen on post - cardiac arrest brain injury in rabbits.Method Sixty New Zealand rabbits were randomly divided into two groups,namely experiment group ( group A,n =30 ) and control group ( group B,n =30 ).Inhalation of 2% hydrogen gas was conferred to rabbits immediately at the end of cardiac arrest modeling for 72 hours in the group A. Air instead was given to rabbits in the group B.Blood samples were collected before cardiac arrest (CA),and 4,12,24 and 72 hours after restoration of spontaneous circulation (ROSC) in all rabbits for determining the levels of hydrogen,tumor necrosis factor - α ( TNF - α),neuron - specific enolase (NSE) and protein S100β.At the same time,rectal temperature,mean arterial pressure,heart rate and respiration rate were recorded,and the neurologic deficit scoring (NDS) was carried out.The rate of systemic inflammatory response syndrome ( SIRS ) and the rate of survival of rabbits were analyzed. Results There was no significant difference in level of TNF - α activation between group A and group B within12 h of cardiopulmonary resuscitation (CPR).In group A,TNF - α level and the rate of SIRS peaked at 24 hours after CPR,which were higher than those in group B,and then decreased gradually,and the rate of survival was higher than that in group B in 72 hours after ROSC,the NSE was lower than that in group B at 24 hours after ROSC.In group B,S100β level began to increase significantly 4 hours after CPR,which was higher than that in group A,the level of NDS in group B was higher than that in group A 72 hours after ROSC.Conclusions Inhalation of hydrogen gas lessened inflammation and alleviated the brain injury after CPR.

  • [1]万智,唐万春.2015心肺复苏指南展望[J].中华急诊医学杂志,2011,20(1):7-10.
  • [2]Laver S,Farrow C,Turner D,et al.Mode of death after admission to an intensive care unit following cardiac arrest[J]. Intensive Care Med,2004,30 (11):2126-2128.
  • [3]沈洪.现代心肺复苏研究的新视点[J].中华急诊医学杂志,2009,18 (1):6-7.
  • [4]张威,蔡建美,康志敏,等.氢分子医学研究进展[J].第二军医大学学报,2009,30(10):1203-1205.
  • [5]蔺际,李欣,李慧,等.经皮电刺激心外膜建立大鼠心搏骤停模型[J].中华急诊医学杂志,2010,19 (7):698-702.
  • [6]Ge P,Zhao J,Li S,et al.Inhalation of hydrogen gas attenuates cognitive impairment in transient cerebral ischemia via inhibition of oxidative stress[J].Neurol Res,2012,34 (2):187-94.
  • [7]Purdy PD,Sr DMD,Batjer HH,et al. Microfibrillar collagen model of canine cerebral infarction[J].Stroke,1989,20 (10):1361-1367.
  • [8]Levy MM,Fink MP,Marshall JC,et al.2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference[J].Intensive Care Med,2003,29:530-538.
  • [9]盛志勇,胡森.多器官功能障碍综合征[M].北京:科学出版社,1999:18-63.
  • [10]李南,张东,王育珊,等.心肺复苏后多器官功能障碍综合征的临床分析[J].中华急诊医学杂志,2010,19(7):680-683.
  • [11]Schneider A,Bottiger BW,Popp E.Cerebral resuscitation after cardiocirculatory arrest[J].Anesth Analg,2009,108 (3):971-979.
  • [12]Peberdy MA,Callaway CW,Neumar RW,et al. Post-cardiac arrest care:2010 American Heart Association Guidelines for cardiopulmonary resuscitation and emergency cardiovascular care[J].Circulation,2010,122 (18 Suppl 3):S768-786.
  • [13]Huang CS,Kawamura T,Toyoda Y,et al.Recent advances in hydrogen research as a therapeutic medical gas[J].Free Radio Res,2010,44 (9):971-982.
  • [14]Hong Y,Chen S,Zhang JM.Hydrogen as a selective antioxidant:a review of clinical and experimental studies[J].J Int Med Res,2010,38 (6):1893-1903.
  • [15]Ohsawa I,Ishikawa M,Takahashi K,et al.Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals[J].Nat Med,2007,13 (6):688-694.
  • [16]Cai J,Kang Z,Liu K,et al.Neuroprotective effects of hydrogen saline in neonatal hypoxia-ischemia rat model[J].Brain Res,2009,1256:129-137.
  • [17]Nakao A,Kaczorowski DJ,Wang Y,et al.Amelioration of rat cardiac cold ischemia/repeffusion injury with inhaled hydrogen or carbon monoxide,or both[J].J Heart Lung Transplant,2010,29 (5):544-553.
  • [18]Kawamura T,Huang CS,Tochigi N,et al.Inhaled hydrogen gas therapy for prevention of lung transplant-induced ischemia/reperfusion injury in rats[J].Transplantation,2010,90 (12):1344-1351.
  • [19]肖敏,杨敬宁,李小燕,等.兔心肺复苏后全身炎症反应综合征的动态变化[J].中华急诊医学杂志,2011,20(8):830-834.
  • [20]李海玲,缪文丽,林慧艳,等.S100蛋白、神经元特异性烯醇化酶对心肺复苏后脑损伤的评估[J].中华急诊医学杂志,2008,17(3):294-297.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn