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Effects of HIF - 1α on mitochondrial function of cardiomyocytes stimulated by lipopolysaccharide

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Author:
No author available
Journal Title:
Chinese Journal of Emergency Medicine
Issue:
8
DOI:
10.3760/cma.j.issn.1671-0282.2012.08.009
Key Word:
脂多糖;缺氧诱导因子-1α;心肌细胞;线粒体;Lipopolysaccharide;Hypoxia inducible factor-1α;Cardiomyocyte;Mitochondrial

Abstract: Objective To investigate the effects of HIF-1α,on mitochondrial function of cardiomyocytes stimulated by lipopolysaccharide (LPS).Methods Cultured neonatal cardiomyocytes were randomly (random number) distributed to four groups (n =5).Control group:the neonatal cardiomyocytes were cultured in normal condition without any treatmeat; LPS group:cardiomyocytes were exposed to 1 μg/ml LPS for 2 h; YC-1 + LPS group:cardiomyocytes were exposed to 4 μmol/L YC-1 for 8 h before LPS treatment; YC-1 group:cardiomyocytes were exposed to 4μmol/L YC-1 for 8 h.As indexes of mitochondrial function,MMP,the content of ATP and the activity of cytochrome oxidase were measured,COX1/2 protein expression was determined by Western blotting.Results Compared with control group,LPS decreased MMP,the content of ATP and the activity of COX obviously ( P <0.05 ),depressed the expression of COX- 1 protein ( P < 0.05 ),induced the expression of COX-2 protein ( P < 0.05 ).Compared with the LPS group,MMP,the content of ATP and the activity of COX were markedly attenuated ( P < 0.01 ),the expression of COX-1 was significantly increased and the expression of COX-2 was decreased in theYC-1 +LPS group (P < 0.01 ).Conclusions YC-1 attributes to the dysfunction of mitochondrial of myocyte by alterating the expression of COX-1 and 2, HIF-1α may attenuate the mitochondrial dysfunction of cadiomyocytes stimulated by LPS.

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