Dynamic changes of ERK1/2 protein in lung fibroblasts of newborn rats with hyperoxia-induced chronic lung disease
- Author:
- No author available
- Journal Title:
- Chinese Journal of Emergency Medicine
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1671-0282.2012.01.008
- Key Word:
- 高氧;新生大鼠;肺成纤维细胞;细胞外信号调节激酶;Hyperoxia;Newborn rat;Lung fibroblast;Extracellular signal regulated protein kinase
Abstract： Objective To investigate dynamic changes of extracellular signal regulated protein kinase (ERK) 1/2 in lung fibroblast of newborn rats with chronic lung disease (CLD) caused by hyperoxia.Methods Full-term newborn rats were randomly divided into two groups:air-exposed group and hyperoxia - exposed with 90％ oxygen group.Rats were sacrificed separately 3 d,7 d and 14 days after exposure to air or 90％ oxygen. Then lung fibroblasts of rats were isolated and primarily cultured. By using Immunocystochemistry,Western-blot and RT-PCR methods,the levels of ERK1/2 protein and expressions of ERK1/2 mRNA were measured. Results The levels of p-ERK1/2 protein in lung fibroblast in the hyperoxia group were significant higher on the 7th day and 14th day after exposure to 90％ oxygen compared with those in the air-exposed group (P ＜0.01 ).And the levels of total ERK1/2 protein and expressions of ERK1/2 mRNA did not change noticeably and were not significantly different between two groups (P ＞0.05 ).Conclusions The activation of phosphorated ERK1/2 may lead to lung fibrosis caused by hyperoxia in newborn rats.
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