Expression of microtubule-associated protein 1 light chain 3 following recurrent neonatal seizures in hippocampus
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1671-0282.2010.06.009
- Key Word:
- 惊厥;新生期;微管相关蛋白1轻链3;免疫印迹;自噬;3-MA;海马;大鼠;Seizure;neonatal;LC-3;Western blot;Autophagy;3- MA;Hippocampus;Rat
Abstract： Objective To explore the repetitive expressions of autophagy marker protein-rnicrotubule-associ-ated protein 1 light chain 3 (LC3) in hippocampus in newborn rats with recurrent seizure and the influence of 3-methyladeine (3-MA) on LC2 expressions. Method Seventy-two 6-day-old SD rats were randomly (random nam-ber) divided into the recurrent neonatal seizure group (RS group, n = 24), the 3-MA-treated seizure group (3-MA group, n = 24) and control group (n = 24). Rats in RS group were subjected to 55 attacks of seizure induced by flurothyl in 9 successive days from the 6th postnatal day (P6). In 3-MA group, 2 μL of 3-MA was injected every day till seizure induced. Western blot analysis was used to determine LC3 protein level in hippocampus at different intervals of 1.5 h,3 h,6 h and 24 h after the last convulsion. The LC3 protein level was analyzed with Dunnett test after ANOVA. Results LC3 protein levels in RS group at the different intervals were significantly higher than those in the control group and in 3-MA group (F =4.70,5.28,8.51 and 5.89, respectively, P <0.05), and there were no significant differences in LC3 protein level between 3-MA group and control group at those intervals (P > 0.05). Conclusions The autophagy/lysosomal pathway is immediately activated after recurrent seizure evidenced by the elevated expressions of LC3 in hippocampus. The 3-MA is involved in the regulation of autophagy/ lysosomal pathway by down-regulating the expressions of LC3.
- [1]倪宏,水泉祥.热惊厥幼龄大鼠脑内Fos和热休克蛋白-70及锌离子转运体3 mRNA表达的研究[J].2008,(1).
- [2]毛定安,刘利群,薄涛,等.川芎嗪对大鼠惊厥性脑损伤caspase-1 mRNA表达的影响[J].2007,(4).
- [3]倪宏,水泉样.热性惊厥与颞叶癫癎[J].2003,(6).
- [4]沈扬,梁立治,洪明晃,等.微管相关蛋白LC3和自噬基因Beclin1在上皮性卵巢癌中的表达及其意义[J].2008,(6).
- [5]倪宏,SHUI Quan-xiang.热惊厥幼龄大鼠脑内Prolactin蛋白及Zinc transporter 1 mRNA表达[J].2008,(8).
- [6]倪宏.发育中脑惊厥性损伤与海马Zn2+转移[J].2006,(4).
- [7]倪宏,姜玉武,丁美丽,等.幼年大鼠青霉素点燃后慢性认知功能缺陷的分子机制研究[J].2009,(4).
- [8]Raman, Sankar,Jong M, Rho.Do seizures affect the developing brain? Lessons from the laboratory.[J].2007,22(5 Suppl).
- [9]Ueno T,Tanida I,Tanida Miyake E,etc.The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification.[J].2003,278(41).
- [10]颜峰平,陈忆九,黄晓华.大鼠皮肤挫伤后Beclin1和LC3的表达[J].2007,(1).
- [11]Gustafsson AB,Gottlieb RA.Autophagy in ischemic heart disease.[J].2009,104(2).
- [12]Rubinsztein DC.The roles of intracellular protein-degradation pathways in neurodegeneration.[J].2006,443(7113).
- [13]David C, Rubinsztein,Marian, DiFiglia,Nathaniel, Heintz,etc.Autophagy and its possible roles in nervous system diseases, damage and repair.[J].2005,1(1).
- [14]Kabeya Y.,Kirisako T.,Mizushima N.,etc.LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing[J].2000,19(21).
- [15]X H, Liang,S, Jackson,M, Seaman,etc.Induction of autophagy and inhibition of tumorigenesis by beclin 1.[J].1999,402(6762).
- [16]Scher MS.Neonatal seizures and brain damage.[J].2003,29(5).
- [17]Reggiori F,Klionsky DJ.Autophagosomes: biogenesis from scratch?[J].2005,17(4).
- [18]Ben-Ari Y,Holmes GL.The neurobiology and consequences of epilepsy in the developing brain.[J].2001,49(3).
- [19]F, Chiricozzi,D, Chieffo,D, Battaglia,etc.Developmental plasticity after right hemispherectomy in an epileptic adolescent with early brain injury.[J].2005,21(11).