Protective effects of hydroxyethyl starch on myocardial ischemia/reperfusion injury in rabbits

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Journal Title:
Volume 19, Issue 04, 2010
Key Word:
Hydroxyethyl starch;Reperfusion injury;Interleukin-8;Endothelin-1;Myeloperoxidase

Abstract: Objective To observe the effects of hydroethyl starch (HES 130/0.4) on serum interleukin-8, endothelin-l(ET-1) and myocardial myeloperoxidase activity (MP0) in rabbits subjected to ischemia-reperfusion, and to explore its possible mechanism. Method Thirty-six rabbits were randomly divided into three groups: lac-tated Ringer's solution group (group A, n= 12), 5% human albumin group (group B, n= 12) and 6% hy-droethyl starch group (group C, n= 12) Hie myodardial ischemia/reperfusion (MI/R) model was established by clamping left anterior descending coronary artery for 30 minutes followed by reperfusion for 180 minutes. Fifteen minutes prior to reperfusion, animals of these 3 given groups were ifused vis right internal jugular vein with 6 ml/kg lactated Ringer'solution, 5% human albumin and hydroethyl starch, respectively. Blood samples were obtained before occlusion (I_0),30 minutes after occlusion (I_1), 60 minutes (R_1) and 180 (R_2) minutes after reperfusion to determine the activities of serum lactate dehydrogenase (LDH), serum creatine phosphokinase (CPK), serum interleukin-8(IL-8) and endotheun-1 (ET-1).The size of myocardial infarction (MI),myocardial water content and myoloperoxidase activity (MPO) were examined as well.Statistical analyses were performed by using SPSS 12.0 software. Multiple comparisons were analyzed by using one-way analysis of variance (SNK- qtest). P < 0.05 was considered statistically significant. Results The serum IDH, CPK,IL-8 and ET-1 weregradually increased after reperfusion, however, 180 min after reperfusion all serum biomarkers in 6% hydroethylstarch group (group C) were significantly lower than those in Iactated Ringer's solution group (group A) [(181 ±17) U/lvs. (334±39) U/L; (1927±205) U/L vs. (2987±326) U/L; (5.03±1.16) ng/L vs. (6.96±1.21) ng/L; (380±78.4) ng/L vs. (667.2±82.1) ng/L. all P <0.05], and than those in 5% human albu-min group (group B) [(181 ±17) U/L vs. (320±38) U/L; (1727 ±205) U/L vs. (2218 ±290) U/L; (5.03 ±1.16) ng/L vs. (5.90±1.03) ng/L; (380.9±78.4) ng/L vs. (615.6±80.2) ng/L, all P <0.05]. Theactivity of MPO of ischemic area in group C (0.20 ± 0.09 ng/L) was significantly lower than that in group A(0.48±0.15 ng/L) and in group B (0.37 ±0.12 ng/L) (P < 0.05). Myocardial water content and infarct sizeof heart found in group C were significantly smaller than those in both group A and group B (P < 0.05). Conclu-sions The 6% hydroethyl starch exhibits its protective effects on myocardial ischemia-reperfusion injury by pre-venting the production and release of serum IL-8 and ET-1, decreasing neutrophil infiltration capillary permeabilityand improving microvascular circulation.

  • [1]Inan N,Iltar S,Surer H,et al.Effect of hydroxyethyl starch 130/0.4on ischaemia/reperfusion in rabbit skeletal muscle[J].Eur J Anaesthe-siol,2009,26(2):160-165.
  • [2]Varga R,T(o)ir(o)k L,Szabó A,et al.Effects of colloid solutions an is-chemia-reperfusion-induced periosteal microcirculatory and inflammatory reactions:comparison of dextran,gelatin,and hydroxyethyl starch[J].Crit Care Med,2008,36(10):2949-2950.
  • [3]Xiong L,Lei C,Wang Q,et al.Acute normovolaemic haemodilution with a novel hydroxyethyl starch (130/0.4) reduces focal cerebral is-chaemic injury in rats[J].Eur J Anaesthesiol,2008,25(7):581-588.
  • [4]Neumann FJ,Ott I,Gawaz M,et al.Cardiac release of cytokines and in-flammatory responses in acute myocardiac infarction[J].Circulation,1995,92(4):748-755.
  • [5]Ren G,Dewald 0,Frangogiannis NG.Inflammatory mechanisms in my-ocardial infarction[J].Curr Drug Targets Inflamm Allergy,2003,2(3):242-256.
  • [6]Takahashi T,Hato F,Yamane T,et al.Activation of human neutrophil by cytokine-activated endothelial cells[J].Circres,2001,88(4):422-429.
  • [7]王吉文,黄建群,王韶颖.大鼠心肌再灌注白介素-8的变化及激素干预的影响[J].中华急诊医学杂志,2001,10(4):244-246.
  • [8]Matharu MM,Butler LM,Rainger GE,et al.Mechanisms of the anti-inflammatory effects of hydroxyethyl starch demonstrated in a flow-basedmodel of neutrophil recruitment by end othelial cells[J].Crit Care Med,2008,36(5)-.1536-1542.
  • [9]Tian J,Lin X,Zhou W,et al.Hydroxyethyl starch inhibits NF-kappa B activation and prevents the expression of inflammatory mediators in endo-toxic rats[J].Ann Clin Lab Sci,2003,33(4):451-458.
  • [10]Rhee P,Wang D,Ruff P,et al.Human neutrophil activation and in-creased adhesion by various resuscitation fluids[J].Crit Care Med,2000,28(1):74-78.
  • [11]Rhee P,Buris D,Kaufmann C,et al.Lactated ringer's solution re-suscitation causes neutrophil activation after hemorrhagic shock[J].J Trauma,1998,44(2):313-423.
  • [12]Garcia-Villalon AL,Amezquita YM,Monge L,et al.Endothelin-1 po-tentiation of coronary artery contraction after ischemia-reperfusion[J].Vascul Pharmacol,2008,48(2/3):109-114.
  • [13]马建群,张蕾,王连文,等.瑞舒伐他对兔心肌-再灌注损伤内皮功能的影响[J].中华急诊医学杂志,2009,18(5):508-511.
  • [14]Rittoo D,Gosling P,Simms MH,et al.The effects of hydroxyethyl starch compared with gelofusine on activated endothelium and the sys-temic inflammatory response following aortic aneurysm repair[J].Eur J Vase Endovasc Surg,2005,30(5):520-524.
  • [15]Zikria BA,Subbarao C,OZ MC,et al.Hydroxyethyl starch macro-molecules reduce myocardial reperfusion injury[J].Arch Surg,1990,125(7):930-934.
  • [16]Rittoo D,Gosling P,Burnley S,et al.Randomized study comparing the effects of hydroxyethyl starch solution with Gelofusine on pulmonary function in patients undergoing abdominal aortic aneurysni surgery[J].Br J Anaesth,2004,93(3):467.
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