High mobility group box-1 protein plays an important role in transcription and expression of interleukin-2 mediated by nuclear factor of activated T cells-2
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF EMERGENCY MEDICINE
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1671-0282.2009.10.018
- Key Word:
- 高迁移率族蛋白B1;活化T细胞核因子;白细胞介素2;细胞因子;小RNA干扰;信号转导;基因表达;免疫;High mobility group box-1 protein;Nuclear factor of activated T cell;Interleukin-2;Cytokine;Small RNA interference;Signal transduction;Gene expression;Immune
Abstract： Obejective To investigate the potential role of high mobility group box-1 protein (HMGB1) in transcription and expression of interleukin (IL)-2 mediated by nuclear factor of activating T cells 2 (NFAT2). Method Firstly, HMGB1 and NFAT2 plasmids were transfected into Hela cells. Doses of HMGB1 plasmid in-creased from 0 to 0.4μg/plate steadily and reporter gene activity of IL-2 was detected and compared. Secondly, endogenous HMGB1 in Hela cells or exogenous HMGB1 in 293T cells was inhibited respectively by sRNAi. Doses of sRNAi for HMGB1 was arisen from 0 to 1.2μg,/plate steadily and reporter gene activity of IL-2 was detected and compared. Results Reporter gene activity of IL-2 increased by 2.12-fold induced by increasing transfection-dose of HMGB1 plasmid in Hela cells (P < 0.01). However, reporter gene activity of IL-2 decreased by 1.7-fold and 4.76-fold repectively due to specific inhibition of exogenous HMGB1 in 293T ceils and endogenous HMGB1 in Hela cells by increasing dose of sRNAi plasmid (P < 0.05 or P < 0.01). Conclusions HMGB1 plays an im-portant role in transcription and expression of IL-2 mediated by NFAT2.
- [1]徐姗,姚咏明,董宁,等.高迁移率族蛋白B1对大鼠脾脏树突状细胞表面共刺激分子表达的影响[J].2006,(8).
- [2]姚咏明,盛志勇.重视对脓毒症本质的探讨[J].2005,(3).
- [3]刘峰,姚咏明,董宁,等.高迁移率族蛋白B1对小鼠巨噬细胞细胞因子和黏附分子表达的影响[J].2008,(2).
- [4]姚咏明,林洪远.高迁移率族蛋白B1在创伤免疫功能紊乱中的作用及其调节机制[J].2008,(9).
- [5]Wang, Haichao,Bloom, Ona,Zhang, Minghuang,etc.HMG-1 as a Late Mediator of Endotoxin Lethality in Mice.[J].1999,285(5425).
- [6]Nakagawa Y,Tamura Y,Takeuchi Y,etc.High mobility group protein-B1 interacts with sterol regulatory element-binding proteins to enhance their DNA binding[J].2005,280(30).
- [7]Lange, SS,Mitchell, DL,Vasquez, KM.High mobility group protein B1 enhances DNA repair and chromatin modification after DNA damage[J].2008,105(30).
- [8]Louis M. Staudt,Anjana Rao,Jena Giltnane,etc.Gene regulation mediated by calcium signals in T lymphocytes[J].2001,2(4).
- [9]Michael Carey,Charina Arayata,Katherine Mitsouras,etc.The DNA Architectural ProteiN HMGB1 Displays Two Distinct Modes of Action That Promote Enhanceosome Assembly[J].2002,22(12).
- [10]Glimcher LH,Ranger AM,Peng SL,etc.NFATc1 and NFATc2 together control both T and B cell activation and differentiation.[J].2001,14(1).
- [11]Fasth AE,Sundberg E,Palmblad K.High mobility group box chromosomal protein 1 acts as a proliferation signal for activated T lymphocytes.[J].2009,214(4).
- [12]J P, Northrop,S N, Ho,L, Chen,etc.NF-AT components define a family of transcription factors targeted in T-cell activation.[J].1994,369(6480).
- [13]Boonyaratanakornkit V,Melvin V,Prendergast P,etc.High-mobility group chromatin proteins 1 and 2 functionally interact with steroid hormone receptors to enhance their DNA binding in vitro and transcriptional activity in mammalian cells.[J].1998,18(8).
- [14]Monticelli S,Rao A,Solymar DC.Role of NFAT proteins in IL13 gene transcription in mast cells.[J].2004,279(35).
- [15]Ross ED,Hardwidge PR,Maher LJ 3rd.HMG proteins and DNA flexibility in transcription activation.[J].2001,21(19).